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Article Abstract
(GIGANTEA) is one of the output key genes for circadian clock in the plant. The gene was cloned and its expression in different tissues was analyzed to facilitate the functional research of . RT-PCR (reverse transcription-polymerase chain reaction) was used to clone gene in present study. This gene was then analyzed by bioinformatics, subcellular localization and gene expression. The coding sequence (CDS) full length of gene was 3 516 bp, encoding 1 171 amino acids with a molecular mass of 128.60 kDa and a theoretical isoelectric point of 6.13. It was a hydrophilic protein. Phylogenetic analysis showed that JrGI of 'Xinxin 2' was highly homologous to GI of . The result of subcellular localization showed that JrGI protein was located in nucleus. The , and genes in female flower buds undifferentiated and early differentiated of 'Xinxin 2' were analyzed by RT-qPCR (real-time quantitative PCR). The results showed that the expression of , and genes were the highest on morphological differentiation, implying the temporal and special regulation of in the differential process of female flower buds of'Xinxin 2'. In addition, RT-qPCR analysis showed that gene was expressed in all tissues examined, whereas the expression level in leaves was the highest. It is suggested that gene plays a key role in the development of walnut leaves.
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