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http://dx.doi.org/10.1097/JS9.0000000000000155 | DOI Listing |
J Med Virol
September 2025
Department of Quantitative Health Sciences, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.
RNA viruses have high mutation frequency, quick generation periods and vast population numbers, which promote fast evolution and host environment adaptation. We integrated scRNA-seq and spatial transcriptomics to profile immune cells and viral gene expression in COVID-19. Cell types and interactions were identified using Seurat-based tools.
View Article and Find Full Text PDFVirus Evol
August 2025
Department of Biomedical Sciences and Pathobiology, 205 Duck Pond Drive, VA-MD College of Veterinary Medicine at Virginia Tech, Blacksburg, VA 24061, United States.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects humans and animals and is therefore a pathogen of grave concern within a One Health framework. Identifying animal-adaptive mutations is critical to preserving One Health, as these mutations could also lead to the persistence of SARS-CoV-2 in animal reservoirs with continual spillover to humans. Therefore, we sought to pair experimental evolution and epidemiological data to identify putative human- and animal-adaptive viral residues and determine their impact on replication-competent SARS-CoV-2 in both human and animal cells.
View Article and Find Full Text PDFVirus Res
August 2025
Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Ch
The emergence of SARS-CoV-2 has posed a substantial global public - health threat and has led to the emergence of diverse variant strains. A prevalent mutation, D614G, is commonly detected in the spike glycoprotein (S) of successive SARS- CoV-2 variants, which enhances viral infectivity. Here, the objective was to examine the influence of mutations on the synthesis and processing of the S protein, virus assembly, and infectivity.
View Article and Find Full Text PDFFront Microbiol
August 2025
Department of Infectious Diseases, West German Centre of Infectious Diseases, University Medicine Essen, University Hospital Essen, University Duisburg-Essen, Essen, Germany.
Introduction: COVID-19, caused by SARS-CoV-2, remains a global health challenge despite the availability of vaccines and antiviral treatments. The emergence of immune escape variants and the persistence of long COVID symptoms continue to complicate prevention and therapy, especially in low-resource settings. Functional inhibitors of acid sphingomyelinase (FIASMAs) have shown promise as broad-spectrum antiviral agents.
View Article and Find Full Text PDFSci Rep
August 2025
Microbiological Analysis Team, Korea Research Institute of Standards and Science (KRISS), Daejeon, 34113, Republic of Korea.
The global spread of SARS-CoV-2 was significantly impacted by the emergence of Variants of Concern (VOC), with Pakistan experiencing similar trends to other countries. To gain a comprehensive understanding of the genomic epidemiology of SARS-CoV-2 strains circulating during Pakistan's third and fourth pandemic waves, we conducted the largest single sequencing effort in the country to date. Using the GridION platform (Oxford Nanopore Technologies), we performed whole genome sequencing on 1052 confirmed COVID-19 patient samples collected from multiple cities across Pakistan between March and October 2021.
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