Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: There is still no consensus on which concentration of mesenchymal stem cells (MSCs) to use for promoting fracture healing in a rat model of long bone fracture.
Aim: To assess the optimal concentration of MSCs for promoting fracture healing in a rat model.
Methods: Wistar rats were divided into four groups according to MSC concentrations: Normal saline (C), 2.5 × 10 (L), 5.0 × 10 (M), and 10.0 × 10 (H) groups. The MSCs were injected directly into the fracture site. The rats were sacrificed at 2 and 6 wk post-fracture. New bone formation [bone volume (BV) and percentage BV (PBV)] was evaluated using micro-computed tomography (CT). Histological analysis was performed to evaluate fracture healing score. The protein expression of factors related to MSC migration [stromal cell-derived factor 1 (SDF-1), transforming growth factor-beta 1 (TGF-β1)] and angiogenesis [vascular endothelial growth factor (VEGF)] was evaluated using western blot analysis. The expression of cytokines associated with osteogenesis [bone morphogenetic protein-2 (BMP-2), TGF-β1 and VEGF] was evaluated using real-time polymerase chain reaction.
Results: Micro-CT showed that BV and PBV was significantly increased in groups M and H compared to that in group C at 6 wk post-fracture ( = 0.040, = 0.009; = 0.004, = 0.001, respectively). Significantly more cartilaginous tissue and immature bone were formed in groups M and H than in group C at 2 and 6 wk post-fracture ( = 0.018, = 0.010; = 0.032, = 0.050, respectively). At 2 wk post-fracture, SDF-1, TGF-β1 and VEGF expression were significantly higher in groups M and H than in group L ( = 0.031, = 0.014; < 0.001, < 0.001; = 0.025, < 0.001, respectively). BMP-2 and VEGF expression were significantly higher in groups M and H than in group C at 6 wk post-fracture ( = 0.037, = 0.038; = 0.021, = 0.010). Compared to group L, TGF-β1 expression was significantly higher in groups H ( = 0.016). There were no significant differences in expression levels of chemokines related to MSC migration, angiogenesis and cytokines associated with osteogenesis between M and H groups at 2 and 6 wk post-fracture.
Conclusion: The administration of at least 5.0 × 10 MSCs was optimal to promote fracture healing in a rat model of long bone fractures.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813838 | PMC |
| http://dx.doi.org/10.4252/wjsc.v14.i12.839 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Biphasic plate-Controlled instability in fracture healing].
Unfallchirurgie (Heidelb)
September 2025
Klinik für Unfall‑, Hand- und Wiederherstellungschirurgie, Universitätsklinikum Münster, Albert-Schweitzer-Campus 1, Gebäude W1, 48149, Münster, Deutschland.
The bony consolidation of fractures depends on various factors. Under optimal conditions fracture healing takes place within a few weeks. An essential requirement for fracture healing is the restoration of adequate biomechanical stability with an interfragmentary movement which is as ideal as possible.
View Article and Find Full Text PDFPreoperative GLP-1 Receptor Agonist Use and Postoperative Outcomes Following Operative Ankle Fracture Repair in Patients With Type 2 Diabetes: A National Database Study.
Foot Ankle Int
September 2025
Department of Orthopaedic Surgery, Medical College of Wisconsin, Milwaukee, WI, USA.
Background: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are increasingly used in management of type 2 diabetes mellitus (T2DM) and obesity. Beyond glycemic control, these agents may influence orthopaedic outcomes. This study aimed to assess the relationship between preoperative GLP-1 RA use and postoperative complications in T2DM patients undergoing operative ankle fracture repair.
View Article and Find Full Text PDFComparative clinical efficacy of nickel-titanium shape memory staples versus miniplate for Bartoníček-Rammelt type III and IV posterior malleolar fractures. A retrospective analysis of 47 consecutive patients treated between January 2022 and June 2024 documented operative time, intraoperative blood loss, fluoroscopy times, healing time, complications, postoperative fracture gap distance (mm), and articular surface step-off (mm) at the ankle joint. Ankle function was assessed using the American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score, the Manchester-Oxford Foot Questionnaire (MOXFQ), and range of motion measurements at 3, 6, 12, and 14 months.
View Article and Find Full Text PDFClinical outcomes of robot-assisted vs. conventional free-hand technique in spine surgery.
Front Surg
August 2025
Department of Orthopedics, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Background: Robot-assisted surgery has been increasingly applied in spinal surgery in recent years, but the differences in efficacy compared to conventional free-hand surgery remain unclear. This study aims to evaluate the impact of these two surgical approaches on spinal surgery patients by analyzing baseline characteristics, surgical data, short-term postoperative outcomes, and long-term functional recovery and pain relief.
Methods: This study first analyzed the differences in baseline characteristics and surgical data between the robot-assisted and conventional free-hand surgery groups, including age, gender, diabetes, hypertension, smoking, and alcohol consumption.
The Role of Sirtuins in Bone Repair From the Perspective of Glucose Metabolism.
Int J Gen Med
September 2025
Department of Cardiothoracic Surgery, Naval Medical Center, Naval Medical University (Second Military Medical University), Shanghai, 200052, People's Republic of China.
Impaired clinical fracture healing remains a major challenge, with surgical treatment often insufficient in patients with metabolic disorders or comorbidities such as diabetes and osteoporosis. Recent advances in metabolomics have brought the Sirtuin protein family to the forefront of bone regeneration research. These NAD⁺-dependent deacetylases exhibit cell-specific expression and regulate critical processes in osteoblasts and osteoclasts, linking glucose metabolism with bone remodeling.
View Article and Find Full Text PDF