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Article Abstract
Bacteriocins are gaining immense importance in therapeutics since they show significant antibacterial potential. This study reports the bacteriocin KAE01 from along with its characterization, molecular modeling, and antibacterial potency, by targeting the matrix protein of . The bacteriocin was purified by using ammonium sulfate precipitation and fast protein liquid chromatography (FPLC), and its molecular weight was estimated as 55 kDa by means of SDS-PAGE. The bacteriocin was found to show stability in a wide range of pH values (2.0-10.0) and temperatures (100 °C for 1 h and 121 °C for 15 min). Antimicrobial screening of the purified peptide against different strains of showed its significant antibacterial potential. Scanning electron microscopy of bacteriocin-induced bacterial cultures revealed significant changes in the cellular morphology of the pathogens. In silico molecular modeling of KAE01, followed by molecular docking of the matrix protein (qSA) of and KAE01, supported the antibacterial potency and SEM findings of this study.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777700 | PMC |
| http://dx.doi.org/10.3390/genes13122333 | DOI Listing |
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