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Correction for 'Characterizing the ligand-binding affinity toward SARS-CoV-2 Mpro physics- and knowledge-based approaches' by Son Tung Ngo , , 2022, https://doi.org/10.1039/d2cp04476e.
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http://dx.doi.org/10.1039/d2cp90233h | DOI Listing |
Anal Chem
September 2025
Laboratory of Organic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland.
DNA-encoded libraries have become widely used in drug discovery, and several different setups to link chemical compounds to DNA have been employed in the field, including single-stranded and double-stranded DNA tags as well as a variety of linker chemistries. In our previous study, we observed distinct differences in binding affinities between ligands coupled either to single-stranded or double-stranded DNA; however, the molecular basis for these differences remained unclear. Here, we present a native ion mobility mass spectrometry approach that incorporates gas- and solution-phase activation techniques to systematically investigate these differences, specifically the impact of DNA tags on binding performance in protein-ligand interactions.
View Article and Find Full Text PDFSmall Methods
September 2025
Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Natural polyreactive antibodies achieve enhanced avidity through heterogeneous ligand binding. However, engineering synthetic heteroligation systems with precise control over recognition motif orientation and distance remains challenging. Here, a DNA framework-based strategy is presented to program heterotypic binding by spatially organizing bivalent aptamers targeting platelet-derived growth factor-BB (PDGF-BB).
View Article and Find Full Text PDFPlatelets
December 2025
Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada.
The integrin family of extracellular matrix (ECM) adhesion receptors plays a central role in platelet function, including adhesion and aggregation. In resting platelets, integrins exist in a low-affinity state for their ligands, and are activated upon ligand binding to the extracellular domain or binding of cytoplasmic proteins such as talin to the intracellular β-tail. Talin function is regulated through autoinhibition, which reduces its integrin-activating function.
View Article and Find Full Text PDFPhytomedicine
August 2025
Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany. Electronic address:
Background: Non-small-cell lung cancer NSCLC is the major diagnosed type of lung cancers in the USA and Europe. It is generally related to poor prognosis and low rates of survival. Oleandrin is a cardiac glycoside occurring naturally in Nerium oleander (Apocynaceae).
View Article and Find Full Text PDFPestic Biochem Physiol
November 2025
Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural
The olfactory system of insects plays a vital role in their survival by enabling them to detect chemical cues and adapt to changing environments. The rape stem weevil, Ceutorhynchus asper, is a significant pest posing a challenge for rapeseed production due to its destructive feeding habit and increasing resistance to insecticides. So far, there's still limited knowledge about structure and function of odorant binding proteins (OBPs) in beetles like C.
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