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Intestinal contents affect the characterization of edible insect bioactive compounds. Two empty intestine methods, namely, traditional static method (TSM) or salt immersion stress method (SISM), associated with extraction solvents water (W), 50 % water-ethanol (W:E) or 100 % ethanol (E), were used to obtain six Protaetia brevitarsis larval extracts. The total flavonoid content (TFC) in the W:E extracts was significantly higher than that in the W and E extracts, with TSM-W:E the highest (p < 0.05). The relative contents of 132 bioactive compounds, especially p-hydroxyphenylacetic acid, citric acid, and dehydroepiandrosterone, were different between TSM-W and SISM-W. TSM-W:E had significantly higher 2,2-diphenyl-1-picrylhydroxy· (DPPḢ) scavenging and pancreatic lipase (PL) inhibitory activity than SISM-W:E (p < 0.05). DPPḢ scavenging and PL inhibitory activities were highly correlated with TFC and carbohydrates, respectively. Thus, bioactive compounds in P. brevitarsis extracts can be obtained selectively using pretreatment methods, which might be beneficial for high-value utilization of P. brevitarsis.
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http://dx.doi.org/10.1016/j.foodchem.2022.134891 | DOI Listing |
J Food Prot
August 2025
Post-Harvest Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Republic of Korea. Electronic address:
In this study, we investigated the microbial quality and bacterial diversity in Protaetia brevitarsis larvae reared on different substrates (spent mushrooms and oak sawdust). The microbiological quality of the rearing substrates differed significantly. Total viable counts (TVC) did not differ between oak sawdust and mushroom-based substrates, whereas coliforms (4.
View Article and Find Full Text PDFInsects
August 2025
Hoxbio, Business Center, Gyeongsang National University, Jinju 52828, Republic of Korea.
The scarab species , an edible insect, has been used in traditional medicine, as animal feed, and for converting agricultural organic wastes into biofertilizer. The intestinal tract, which contains a diverse array of microbiota, including viruses, plays a critical role in animal health and homeostasis. We previously conducted a comparative analysis of the gut microbiota of third-instar larvae of obtained from five different farms and found significant differences in the composition of the gut bacterial microbiota between farms.
View Article and Find Full Text PDFSci Total Environ
August 2025
School of Ecological and Environmental Sciences, Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, East China Normal University, Shanghai 200241, China; Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai
Polyethylene (PE) microplastics (MPs), derived primarily from agricultural mulch films and other plastic debris, remain significant soil contaminants due to their resistance to degradation. This study reveals that soil-dwelling grubs (Protaetia brevitarsis larvae) possess a unique ability to degrade PE MPs with ultrahigh molecular weight (> 600 kDa) through synergistic interactions with gut microbes. Over a 28-day experimental period, the larvae consumed an average of 62.
View Article and Find Full Text PDFJ Agric Food Chem
August 2025
School of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.
While thiol-reactive compounds have been extensively utilized by plants as defensive metabolites, prior investigations into these natural products remained limited. We employed a reactivity-directed analysis method utilizing high-resolution mass spectrometry to conduct thiol reactome profiling on extracts from 70 different plant-derived foods, resulting in the tentative prediction of chemical formulas of 71 abundant molecular adduct ions. Then, we selected a prominent adduct ion for structural elucidation, leading to the identification of ()-dihydromaleimide (DHM) and ()-dihydromaleimide β-d-glucoside (DHMG) in snow pea ().
View Article and Find Full Text PDFEcol Evol
August 2025
Key Laboratory of Economic and Applied Entomology of Liaoning Province, College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China.
Determining the ecological role of insect larvae is crucial for pest control and resource utilization, which usually relies on accurate species identification. White grubs, the larvae of Scarabaeoidea, exhibit highly diverse behaviors but share a similar morphology, making identification challenging. In this study, an improved six-armed olfactometer was used to test the feeding preferences of two sympatric white grubs, and .
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