Simulation of a tumor cell flowing through a symmetric bifurcated microvessel.

Microvessel bifurcations serve as the major sites of tumor cell adhesion and further extravasation. In this study, the movement, deformation, and adhesion of a circulating tumor cell flowing in a symmetric microvessel with diverging and converging bifurcations were simulated by dissipative particle dynamics combined with a spring-based network model. Effects of the initial position of the CTC, externally-applied acceleration and the presence of RBCs on the motion of the CTC were investigated. The results demonstrated that the CTC released at the centerline of the parent vessel would attach to the vessel wall when arriving at the apex of diverging bifurcation and slide into the daughter branch determined by its centroid deflection and finally form firm adhesion at relatively lower flow rates. As the external acceleration increases, the increasing shear force enlarges the contact area for the adherent CTC on the one hand and reduces the residence time on the other hand. With the presence of RBCs in the bloodstream, the collision between the adherent tumor cell at the diverging bifurcation and flowing RBCs promotes the firm adhesion of CTC at lower flow rates.