Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Monitoring biosynthesis activity at single-cell level is key to metabolic engineering but is still difficult to achieve in a label-free manner. Using hyperspectral stimulated Raman scattering imaging in the 670-900 cm region, localized limonene synthesis are visualized inside engineered Escherichia coli. The colocalization of limonene and GFP-fused limonene synthase is confirmed by co-registered stimulated Raman scattering and two-photon fluorescence images. The finding suggests a limonene synthesis metabolon with a polar distribution inside the cells. This finding expands the knowledge of de novo limonene biosynthesis in engineered bacteria and highlights the potential of SRS chemical imaging in metabolic engineering research.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661820 | PMC |
| http://dx.doi.org/10.1002/advs.202203887 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antibacterial mode of action of thyme white (Thymus vulgaris L.) essential oil and its constituents, thymol and carvacrol against Agrobacterium tumefaciens via down-regulation of manganese transport genes, sitABCD and mntH.
Pestic Biochem Physiol
November 2025
Department of Agriculture, Forestry and Bioresources, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of
In this study, we evaluated the antibacterial activities of plant essential oils (EOs) from the Lamiaceae family against Agrobacterium tumefaciens to find new eco-friendly antimicrobials. Thymus vulgaris L. (thyme white) EO demonstrated the most potent fumigant antibacterial activity among these.
View Article and Find Full Text PDFTuning the Upper Critical Solution Temperature of the Polystyrene-Terpineol System toward a Low-Temperature Membrane Formation.
ACS Omega
August 2025
Chemical and Materials Engineering Department, Pigman College of Engineering, University of Kentucky, Lexington, Kentucky 40506, United States.
The upper critical solution temperature (UCST) of binary polymer-solvent blends can create porous structures for many applications, including filtration. This study investigates how UCST can be tuned in a non-aqueous system of polystyrene and terpineol. The addition of small molecules, γ-valerolactone, oleic acid, and limonene, was tested to modify the terpineol-polystyrene phase separation temperature.
View Article and Find Full Text PDFChemotaxonomy and Bioactivity of Cupressus sempervirens Varieties Essential Oils: Insights Into Their Antioxidant and Enzyme Inhibitory Potential.
Chem Biodivers
August 2025
Chemical, Pharmacological and Gallenic Development Laboratory, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
Collagenase and tyrosinase are key enzymes involved in skin aging and melanin synthesis, making the search for effective natural inhibitors increasingly important in cosmetic and pharmaceutical fields. In this context, the present study explores the chemotaxonomy, antioxidant capacity, and enzyme inhibitory potential of essential oils (EOs) from three Tunisian varieties of Cupressus sempervirens: var. horizontalis, var.
View Article and Find Full Text PDFChemical Constituents, In Silico Studies and In Vitro Antioxidant, Enzyme Inhibitory and Antibacterial Activities of the Algerian Essential Oil and Extracts.
Plants (Basel)
August 2025
Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Espinardo, 30071 Murcia, Spain.
The objective of this study is to evaluate, for the first time, the chemical composition and the antioxidant, enzyme inhibitory, photoprotective and antibacterial properties of the essential oil (EO) as well as its organic extracts. The analysis of the EO obtained from the aerial parts of was carried out employing the technique of gas chromatography with flame ionization detection (GC-FID) and mass spectrometry (GC-MS). Forty-four constituents were identified, constituting 91.
View Article and Find Full Text PDFChemical profile of essential oil from Pimenta racemosa leaves, antioxidant potential, and its enzyme inhibitory properties.
Sci Rep
August 2025
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo, 11566, Egypt.
Pimenta racemosa var. recemosa (Mill.) J.
View Article and Find Full Text PDF