Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The simultaneous detection of three kinds of small-molecule contaminants (antibiotics, mycotoxins, and hormones) in milk was realized by using an 8-17 DNAzyme-based fluorescent enzyme-linked immunosorbent assay (ELISA), in which 8-17 DNAzyme was utilized as the catalytic enzyme for amplifying the signal. Compared with the conventional ELISA in which horseradish peroxidase is used as the catalyzing factor, this 8-17 DNAzyme-based ELISA could achieve multicolor signal output with lower detection limits. The linearities for chloramphenicol, 17β-estradiol, and aflatoxin M were in the range of 0.3 ng/mL-3 μg/mL, 3 ng/mL-3 μg/mL, and 3 pg/mL-3 ng/mL with quantitation limits of 0.3, 3, and 0.003 ng/mL, respectively. This proposed scheme demonstrated that the 8-17 DNAzyme might be an effective substitute for horseradish peroxidase in ELISA for the development of ultrasensitive and multicolor fluorescence immunoassay, which would stimulate the development of ELISA in a new orientation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.2c03833 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Modular Engineering of the DNAzyme-Based Electrochemiluminescence Biosensor with the Nitrogen and Sulfur Codoped Carbon Dots as Near-Infrared Emitters for MicroRNA detection.
Anal Chem
May 2025
College of Chemistry and Chemical Engineering, Precise Synthesis and Function Development Key Laboratory of Sichuan Province, Institute of Applied Chemistry, China West Normal University, Nanchong, Sichuan637000, China.
DNAzyme-based biosensors remain at the forefront of microRNA (miRNA) analysis efforts. In this work, an electrochemiluminescence (ECL) biosensor technology integrating miRNA-initiated DNAzyme with a near-infrared ECL emission system was designed to detect miRNA. The DNAzyme was designed through the rational reconstitution of Mg-specific 8-17 DNAzyme.
View Article and Find Full Text PDFThe 8-17 DNAzyme can operate in a single active structure regardless of metal ion cofactor.
Nat Commun
May 2024
Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704, Poznań, Noskowskiego, 12/14, Poland.
DNAzymes - synthetic enzymes made of DNA - have long attracted attention as RNA-targeting therapeutic agents. Yet, as of now, no DNAzyme-based drug has been approved, partially due to our lacking understanding of their molecular mode of action. In this work we report the solution structure of 8-17 DNAzyme bound to a Zn ion solved through NMR spectroscopy.
View Article and Find Full Text PDFRatiometric Detection of Zn Using DNAzyme-Based Bioluminescence Resonance Energy Transfer Sensors.
Chemistry (Basel)
September 2023
Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA.
While fluorescent sensors have been developed for monitoring metal ions in health and diseases, they are limited by the requirement of an excitation light source that can lead to photobleaching and a high autofluorescence background. To address these issues, bioluminescence resonance energy transfer (BRET)-based protein or small molecule sensors have been developed; however, most of them are not highly selective nor generalizable to different metal ions. Taking advantage of the high selectivity and generalizability of DNAzymes, we report herein DNAzyme-based ratiometric sensors for Zn based on BRET.
View Article and Find Full Text PDFHighly sensitive detection of Pb in the environment with DNAzyme and rolling circle amplification reaction.
Spectrochim Acta A Mol Biomol Spectrosc
April 2024
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China; Key Laboratory of Energy Catalysis and Conversion of Nanchang, Nanchang 330022, PR China. Electronic address:
Lead (Pb) is a toxic heavy metal that can severely pollute the environment and cause harm to public health. Therefore, the prompt and accurate monitoring of lead levels in the environment is vital. In this study, a novel DNAzyme-based cascade signal amplification biosensor that could detect Pb with high sensitivity was designed through the combination of the strand displacement reaction (SDR) and rolling circle amplification (RCA).
View Article and Find Full Text PDFSpatiotemporal control of DNAzyme activity for fluorescent imaging of telomerase RNA in living cells.
Anal Chim Acta
January 2024
College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou University, Zhengzhou, 450001, China; The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. Electronic address:
Background: Human telomerase is a ribonucleoprotein complex that includes proteins and human telomerase RNA (hTR). Emerging evidence suggested that the expression level of hTR was high related with the development of tumor, so it is important to accurately detect the content of hTR. Optical control of DNAzyme activity shows a promising strategy for precise biosensing, biomedical imaging and modulation of biological processes.
View Article and Find Full Text PDF