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The effects of the natural pesticides, phenazines, were reported to be limited by some tolerant metabolism processes within . Our previous studies suggested that the functional cytochrome bc1 complex, the indispensable component of the respiration chain, might participate in tolerating phenazines in . In this study, the cytochrome bc1 mutants of pv. () and pv. (), which exhibit different tolerance abilities to phenazines, were constructed, and the cytochrome bc1 complex was proven to partake a critical and conserved role in tolerating phenazines in . In addition, results of the cytochrome mutants suggested the different functions of the various cytochrome proteins in and that the electron channeled by the cytochrome bc1 complex to cytochrome C4 is the key to reveal the tolerance mechanism. In conclusion, the study of the cytochrome bc1 complex provides a potential strategy to improve the activity of phenazines against .
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http://dx.doi.org/10.1021/acs.jafc.2c03317 | DOI Listing |
Pest Manag Sci
September 2025
National Pesticide Engineering Research Center, State Key Laboratory of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, People's Republic of China.
Background: Rapid advances in generative artificial intelligence (AI) are accelerating the process of pesticide development. However, transfer learning-based de novo design focuses on generating molecules that are highly similar to existing inhibitors, which may limit the exploration of novel scaffolds and thereby constrain innovative breakthroughs in pesticide development.
Results: This study proposes a new strategy for fungicide design using antibiotics.
Eur J Heart Fail
September 2025
School of Cardiovascular & Metabolic Medicine and Science, James Black Centre, King's College London British Heart Foundation Centre of Excellence, London, UK.
Aims: Skeletal muscle energetic augmentation might be a mechanism via which intravenous iron improves symptoms in heart failure, but no direct measurement of intrinsic mitochondrial function has been performed to support this notion. This molecular substudy of the FERRIC-HF II trial tested the hypothesis that ferric derisomaltose (FDI) would improve electron transport chain activity, given its high dependence on iron-sulfur clusters which facilitate electron transfer during oxidative phosphorylation.
Methods And Results: Vastus lateralis skeletal muscle biopsies were taken before and 2 weeks after randomization.
Pestic Biochem Physiol
November 2025
Department of Plant Protection, Faculty of Agriculture, Ankara University, Dıskapı, 06110 Ankara, Türkiye. Electronic address:
Acequinocyl and bifenazate are widely used acaricides that inhibit mitochondrial electron transport at complex III, due to their high efficacy and low side effects. However, resistance development has been reported in Tetranychus urticae populations worldwide, likely as a result of frequent applications. This study assessed the phenotypic resistance levels of T.
View Article and Find Full Text PDFBiomolecules
August 2025
Yanbian University College of Medicine, Yanbian University Hospital, Yanji 133000, China.
Hydrogen sulfide (HS) has been established to regulate mitochondrial respiration and ATP production, but whether the regulation is through -sulfhydration (-SSH) of mitochondrial complexes is not well understood. Recently, HS is known to exert diverse and dose-dependent effects on mitochondrial complexes. However, the involvement of -sulfhydration of each mitochondrial complex and the activities in diabetic hearts have not been revealed.
View Article and Find Full Text PDFEur J Immunol
July 2025
Laboratory of Translational Immunomodulation, VIB Center for Inflammation Research (IRC), Hasselt University, Diepenbeek, Belgium.
Regulatory FOXP3 T cells (Tregs) have been characterized with unique metabolic demands, preferentially relying on fatty acid β-oxidation (FAO) and oxidative phosphorylation (OXPHOS). Several studies have indicated that Treg mitochondrial fitness is crucial for maintaining their stability and suppressive activity with an emphasis on complex-III of the electron transport chain (ETC). Dysfunctional Tregs isolated from patients with autoimmunity like multiple sclerosis (MS) show diminished mitochondrial respiration and the induction of a T helper (Th)1-like phenotype, characterized by increased production of interferon (IFN)-γ.
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