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http://dx.doi.org/10.1097/AOG.0000000000004754 | DOI Listing |
J Vis Exp
August 2025
Department of Nutritional Sciences, University of Wisconsin-Madison;
The retinol isotope dilution (RID) test is the most sensitive method to assess vitamin A status by estimating total liver reserves, considered the reference standard. For gas chromatography-combustion-isotope ratio mass spectrometry detection, C is added to the retinol moiety. The synthetic procedure for C-retinyl acetate begins with the naturally occurring β-ionone.
View Article and Find Full Text PDFFront Cell Infect Microbiol
September 2025
Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Introduction: leaves (FSL), a traditional Chinese ethnomedicinal herbal material used to prepare health-promoting infusions and pharmacologically noted for their robust anti-inflammatory, antioxidant, and broad-spectrum antiviral activities, nevertheless have an as-yet-uncharacterized molecular mechanism of action against human adenovirus (HAdV).
Methods: Ultra-high-performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive-Orbitrap/MS) was employed to identification of FSL components. Publicly available GEO datasets were mined to identify HAdV-associated differentially expressed genes (DEGs).
Carbohydr Polym
November 2025
Food & Nutritional Sciences Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin 999077, Hong Kong, China. Electronic address:
Starch-tannic acid complex is a type of resistant starch, while the influence of these complexes with different starch chain-length distributions (CLDs) on gut microbiota is unclear. Therefore, starch-tannic acid complexes were prepared from five commercial starches with diverse CLDs, and their influence on the gut microbiota was explored using in vitro fermentation with human fecal microbiota. For the first time, results showed that wheat and corn starch-tannic acid complexes significantly promoted propionate production (p < 0.
View Article and Find Full Text PDFCureus
August 2025
Orthopedics and Traumatology, Laiko General Hospital of Athens, Athens, GRC.
The Kuntscher nail, once a pioneering solution for femoral shaft fracture fixation, has largely fallen out of use with the emergence of modern interlocking intramedullary systems. However, retained Kuntscher nails may still be encountered in older patients and can pose significant technical challenges when removal is required, especially in cases of incarceration. Standard closed extraction methods are often ineffective, necessitating alternative surgical strategies.
View Article and Find Full Text PDFEur Spine J
September 2025
Peking University Third Hospital, Beijing, China.
Purpose: In patients presenting with the sandwich fusion, characterized by C1 occipitalization and C2-C3 non-segmentation, leading to stress concentration at the atlantoaxial joint, there is an increased likelihood of atlantoaxial dislocation (AAD). The decision to proceed with transoral surgery is contingent upon the outcomes of intraoperative traction assessment. The complexity of intraoperative decision-making introduces a degree of uncertainty in preoperative planning, complicating both the surgical preparation and doctor-patient communication.
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