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The effect of the addition of different levels of S. carnosus protease (0, 0.15, 0.30, 0.45 and 0.60 g/kg raw meat) on the proteolysis, quality characteristics, and flavor development of Harbin dry sausage was investigated. The results showed that the S. carnosus protease addition to Harbin dry sausage effectively promoted the degradation of meat proteins into peptides and free amino acids, thus resulting in tenderization and inhibiting fat oxidation. Moreover, the S. carnosus protease addition could promote the development of key flavor compounds such as some ketones, acids and esters. Sausage with S. carnosus protease levels of 0.45 g/kg exhibited the most attractive sensory attributes. Molecular docking showed that the S. carnosus protease can interact with myosin heavy chains. In summary, the S. carnosus protease addition can improve quality characteristics and flavor profile of Harbin dry sausage.
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http://dx.doi.org/10.1016/j.meatsci.2022.108827 | DOI Listing |
Food Funct
August 2025
College of Food Science and Technology, Bohai University, No. 19, Keji Rd., New Songshan District, Jinzhou 121013, Liaoning Province, China.
This study aims to enhance the swallowing characteristics of fermented meat pastes by improving their texture and flavor through the targeted inoculation of different starter cultures. () and () were each combined with () or () to prepare fermented meat pastes. The LS ( + ) exhibited significantly higher protease activity, which reduced hardness, gumminess, and chewiness ( < 0.
View Article and Find Full Text PDFBiochem Biophys Res Commun
May 2023
Department of Protein Science, KTH - Royal Institute of Technology, SE-106 91, Stockholm, Sweden. Electronic address:
Within the field of combinatorial protein engineering there is a great demand for robust high-throughput selection platforms that allow for unbiased protein library display, affinity-based screening, and amplification of selected clones. We have previously described the development of a staphylococcal display system used for displaying both alternative-scaffolds and antibody-derived proteins. In this study, the objective was to generate an improved expression vector for displaying and screening a high-complexity naïve affibody library, and to facilitate downstream validation of isolated clones.
View Article and Find Full Text PDFFoods
September 2022
Unità Operativa di Lecce, Istituto di Scienze delle Produzioni Alimentari, Consiglio Nazionale delle Ricerche, 73100 Lecce, Italy.
For centuries, macroalgae, or seaweeds, have been a significant part of East Asian diets. In Europe, seaweeds are not considered traditional foods, even though they are increasingly popular in Western diets in human food applications. In this study, a biological processing method based on semi-solid fermentation was optimized for the treatment of the seaweed .
View Article and Find Full Text PDFMeat Sci
July 2022
College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address:
The effect of the addition of different levels of S. carnosus protease (0, 0.15, 0.
View Article and Find Full Text PDFJ Food Sci
May 2021
College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
The characteristics of the extracellular protease, produced by Staphylococcus carnosus RT6 isolated from Harbin dry sausages, and its hydrolysis of meat proteins were investigated. The protease was purified by ammonium sulfate, ion exchange, and gel filtration chromatography to obtain a 20.0 kDa extracellular protease.
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