Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Gold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. Gold nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as an improved electrochemical platform and for DNA probe immobilization. Azure A is used as an electrochemical indicator of the hybridization event. The biosensor detects either single stranded DNA or RNA sequences of SARS-CoV-2 of different lengths, with a low detection limit of 22.2 fM. In addition, it allows to detect point mutations in SARS-CoV-2 genome with the aim to detect more infective SARS-CoV-2 variants such as Alpha, Beta, Gamma, Delta, and Omicron. Results obtained with the biosensor in nasopharyngeal swab samples from COVID-19 patients show the possibility to clearly discriminate between non-infected and infected patient samples as well as patient samples with different viral load. Furthermore, the results correlate well with those obtained by the gold standard technique RT-qPCR, with the advantage of avoiding the amplification process and the need of sophisticated equipment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974806 | PMC |
| http://dx.doi.org/10.1007/s00604-022-05272-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Correction: Temperature Sensitive PNIPAm-g-PEI/Gold Nanotriangle for Gene Delivery Promotion.
Mol Biotechnol
August 2025
Shandong Provincial Engineering Research Center of Novel Pharmaceutical Excipients, Sustained and Controlled Release Preparations, College of Medicine and Nursing, Dezhou University, Dezhou, 253023, China.
Amino Acid-Mediated Preparation of Highly Dendritic Chiral Plasmonic Nanoparticles for SERS-Based Enantiomer Recognition.
ACS Appl Mater Interfaces
July 2025
Nanjing Research Center for Biomedical Electron Microscopy (NRC-BEM), Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210093, P. R. China.
Chiral plasmonic nanoparticles (CPNPs) are blooming building blocks in modern nanotechnology and have attracted great attention due to their unique capabilities in manipulating light, enabling enantiomeric-based theranostics, realizing chiral sensing, and so on. Here, we report a strategy for the preparation of highly dendritic CPNPs. Using gold nanotriangles as seeds and cysteine as a shape-directing agent, two distinct dendritic nanostructures, termed gold nanoflowers (Au NFs) and gold nanourchins (Au NUs), can be obtained by adjusting the cysteine concentration within the reaction system.
View Article and Find Full Text PDFGold nanotriangle-enhanced high-sensitive determination of alpha-fetoprotein via lateral flow immunoassay in serum.
Mikrochim Acta
June 2025
School of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, China.
The first visual lateral flow immunoassay (LFIA) is reported using gold nanotriangles (AuNTs) as labeling materials for high-sensitivity alpha-fetoprotein (AFP) detection in serum. Compared with traditional gold nanoparticles, the use of anisotropic AuNTs significantly reduces the detection limit of AFP due to larger surface area and strong localized surface plasmon resonance, which can reach 5 ng/mL in serum samples with naked eyes. Under optimized conditions, this method demonstrates excellent accuracy and reproducibility in both buffer and serum systems.
View Article and Find Full Text PDFMorphology-Preserved Alloying in Anisotropic Gold Nanoparticles Using Atomically Precise Nanoclusters.
Small
May 2025
Department of Chemistry, DST Unit of Nanoscience (DST UNS) and Thematic Unit of Excellence (TUE), Indian Institute of Technology Madras, Chennai, 600 036, India.
Noble metal nanoparticles (NPs) exhibit superior plasmonic, catalytic, electronic, and magnetic properties upon alloying with a second metal. However, the synthesis of bimetallic alloy NPs of non-spherical morphologies presents a challenge due to the necessity of concurrently modulating the nucleation and growth kinetics of various metallic constituents. In this study, a simple solution-phase reaction between a phosphine-protected copper nanocluster (NC), namely [Cu(DPPE)H] [DPPE = 1,2-bis(diphenylphosphino)ethane] (abbreviated as Cu) and gold nanotriangles (AuNTs) is reported as a straightforward strategy to obtain gold-copper alloy nanotriangles (AuCuNTs) while keeping their sizes and sharp edges intact.
View Article and Find Full Text PDFToxicological Comparison between Gold Nanoparticles in Different Shapes: Nanospheres Exhibit Less Hepatotoxicity and Lipid Dysfunction and Nanotriangles Show Lower Neurotoxicity.
ACS Omega
October 2024
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Article Synopsis
- Gold nanoparticles (AuNPs) of different shapes, specifically spherical (AuS) and triangular (AuT), were studied to understand their toxic effects on various organs in mice, particularly in relation to plastic particle exposure.
- The study revealed that triangular AuNPs (AuT) led to significantly increased levels of certain biomarkers related to liver and cholesterol metabolism compared to spherical AuNPs (AuS), which showed higher levels of nitric oxide.
- Overall, the findings suggest that spherical AuNPs may pose a lower risk for liver toxicity and lipid metabolism issues, while triangular AuNPs may be less neurotoxic, providing insights for future therapeutic applications and organ protection strategies.