98%
921
2 minutes
20
We developed a multiplex real-time PCR assay with amplicon melting curve analysis to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay should be a valuable tool for identification of potentially toxigenic C. ulcerans.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.mimet.2022.106454 | DOI Listing |
Antibiotics (Basel)
August 2025
Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil.
Background: is an emerging zoonotic pathogen capable of cau-sing diphtheria-like infections in humans.
Objectives: we report, for the first time in Brazil, the detection and phenotypic/genomic characterization of three atoxigenic ST-339 strains isolated from domestic animals, including one with a ciprofloxacin resistance profile linked to double GyrA mutations (S89L, D93G).
Methods: species identification was performed by MALDI-TOF MS, followed by in vitro antimicrobial susceptibility testing, whole-genome sequencing, and bioinformatic analyses to predict virulence determinants, antimicrobial resistance genes, CRISPR-Cas systems, mobile genetic elements, and in silico structural analysis as well as phylogenetic reconstruction.
J Am Assoc Lab Anim Sci
June 2025
Harvard Center for Comparative Medicine, Harvard Medical School, Boston, Massachusetts.
Cranially-implanted macaques are a crucial model for neuroscience research. Implant complications include abscesses, meningoencephalitis, and implant-tissue margin infections. Antimicrobial overuse has increased bacterial resistance, risking macaque health and complicating treatment of infections.
View Article and Find Full Text PDFNat Commun
May 2025
Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, Paris, France.
Corynebacterium ulcerans is an emerging zoonotic pathogen which causes diphtheria-like infections. Although C. ulcerans is found in multiple domestic and wild animal species, most human cases are linked with pets.
View Article and Find Full Text PDFAntibiotics (Basel)
February 2025
National Reference Center for Toxigenic Corynebacteria, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel (UZ Brussel), Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium.
: Despite a significant reduction in diphtheria incidence and mortality due to vaccination, antitoxin therapy and antibiotic treatments, a concerning resurgence is occurring in Europe. Resistance to penicillins and macrolides is emerging, resulting in a growing challenge for diphtheria management. This retrospective study aims to evaluate and compare antibiotic susceptibilities of both toxigenic and .
View Article and Find Full Text PDFInfection
June 2025
WHO Collaborating Centre for Diphtheria, German National Consiliary Laboratory for Diphtheria, Bavarian Health and Food Safety Authority, Veterinaerstrasse 2, Oberschleißheim, 85764, Germany.
Purpose: Historically, a considerable number of diphtheria outbreaks, mainly in the UK and the USA, have been epidemiologically or bacteriologically linked to the consumption of milk and dairy products. However, with the introduction of milk pasteurization and hygienic improvements in livestock farming and animal welfare, no cases of milk-linked human diphtheria outbreaks or bovine mastitis due to toxigenic corynebacterial have been reported in the recent decades. Here we report the first isolation of a toxigenic Corynebacterium ulcerans strain from the milk of a cow with acute mastitis within nearly 40 years and outside of UK or Finland.
View Article and Find Full Text PDF