Prediction and evaluation of multi epitope based sub-unit vaccine against .

is the most common enteric pathogen in humans and animals. Consumption of contaminated food or water triggers inflammation that allows to spread into the gut and causes gastrointestinal diseases. The infection spreads by intestinal invasion, phagocyte internalization and subsequent dissemination in many other patients. This research used TolA, a membrane protein, to computationally design a multi-epitope vaccine against the pathogen. Complete consistency of the candidate vaccine was checked , and molecular dynamics simulations confirmed the vaccine's stability. According to docking report, the vaccine has a good affinity with toll-like receptors. cloning and codon optimization techniques improved the vaccine's efficacy in manifestation process. The candidate vaccine induced an efficient immune response, as determined by immune simulation. Computational studies revealed that the engineered multi-epitope vaccine is structurally stable, capable of eliciting particular immunological reactions, and therefore a candidate for a latent vaccine. However, wet lab studies and further investigations are required to confirm the results.