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Insulin (INS, hydrophilic) and quercetin (Q, hydrophobic) have broad biological benefits; however, their application is limited because of their instability and poor bioaccessibility. Thus, in this study, water-in-oil-in-water (W/O/W) double emulsion gels based on black bean protein-sodium alginate Maillard conjugate-stabilized double emulsions were fabricated using three different inducers. The water holding capacity (WHC), intermolecular interaction forces, INS and Q encapsulation efficiencies (EEs), rheological and textural properties, microstructures, and structures were influenced by both inducer type [CaCl, glucolactone (GDL), and glutamine transaminase (TGase)] and concentration. The addition of each inducer significantly increased the WHC, INS, and Q EEs, and textural properties of the emulsion gel. Moreover, the rheological behavior analysis showed that all the emulsion gels exhibited marked elastic behavior. These results will help develop an emulsion gel functional carrier based on a black soybean protein-carbohydrate-stabilized W/O/W double emulsion for the targeted and sustained release of drugs.
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http://dx.doi.org/10.1016/j.foodchem.2022.132166 | DOI Listing |
Mater Today Bio
October 2025
Department of Trauma and Microreconstructive Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Large bone defects present significant clinical challenges, with distraction osteogenesis (DO) requiring prolonged treatment periods and yielding suboptimal outcomes. Calcitonin gene-related peptide (CGRP) demonstrates potent bone-forming activity but suffers from rapid degradation and a short half-life, limiting its therapeutic applications. This study engineered sustained-release CGRP microspheres using poly(D,L-lactide-co-glycolide)/nano-hydroxyapatite/graphene oxide (PLGA/nHA/GO) composite matrices via W/O/W double emulsion-solvent evaporation method to address these limitations.
View Article and Find Full Text PDFMolecules
July 2025
Biologie du Fruit et Pathologie, INRAE, Univ. Bordeaux, UMR 1332, F-33140 Villenave d'Ornon, France.
The production of water-in-water emulsion droplets, the coalescence of which is prevented by adding oil-in-water micrometric droplets, is reported. Hexadecane (O) and cetyl trimethyl ammonium bromide (CTAB) were added to a W/W emulsion made of dextran (Dex)-enriched droplets in a Polyethyleglycol (PEG)-enriched continuous phase, and the mixture was further sonicated. Using Nile red to label the oil droplets enabled the observation of their presence at the surface of Dex droplets (5 µm), allowing for stabilizing them, preventing coalescence of the W/W emulsion, and mimicking W/O/W double emulsions.
View Article and Find Full Text PDFAAPS PharmSciTech
July 2025
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research-Ahmedabad (NIPER-A), Gandhinagar, Gujarat, 382355, India.
The Food and Drug Administration of the United States (USFDA) and the European Medicines Agency (EMA) have approved peptide-based long-acting formulations to manage chronic diseases. However, the formulation of long-acting injectables for hydrophilic drug molecules and peptides remains a persistent challenge due to poor encapsulation and high initial burst release, which increases the complexity of the formulation. In the present research, we formulated long-acting injectable microspheres for liraglutide, a peptide used for managing type 2 DM (Diabetes Mellitus), utilizing a modified solid-oil-water (S/O/W) method.
View Article and Find Full Text PDFGels
June 2025
Department of Pathology, Medical School of Nantong University, Nantong 226001, China.
The aim of this study was to develop double emulsions-filled chitosan hydrogel beads for topical application and to elucidate their skin penetration behavior. Double emulsions were prepared by a two-step emulsification method, and double emulsions-filled chitosan hydrogel beads were prepared by the extrusion method. The structure, stability, and skin penetration behavior were investigated.
View Article and Find Full Text PDFJ Sci Food Agric
July 2025
College of Food Science and Engineering, Tianjin University of Science and Technology (TUST), Tianjin, China.
Background: Probiotics are susceptible to both internal and external environments.
Methods: Therefore, in this study, we developed two probiotic delivery systems comprising transglutaminase (TGase)-crosslinked water-in-oil-in-water (W/O/W) emulsion microcapsules and sodium alginate gel beads to enhance gastrointestinal resistance and storage stability.
Results: A W/O/W emulsion was fabricated via a two-step emulsification process.