Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Agrobacterium tumefaciens is considered a prominent phytopathogen, though most isolates are nonpathogenic. Agrobacteria can inhabit plant tissues interacting with other microorganisms. Yeasts are likewise part of these communities. We analyzed the quorum sensing (QS) systems of A. tumefaciens strain 6N2, and its relevance for the interaction with the yeast Meyerozyma guilliermondii, both sugarcane endophytes. We show that strain 6N2 is nonpathogenic, produces OHC8-HSL, OHC10-HSL, OC12-HSL and OHC12-HSL as QS signals, and possesses a complex QS architecture, with one truncated, two complete systems, and three additional QS-signal receptors. A proteomic approach showed differences in QS-regulated proteins between pure (64 proteins) and dual (33 proteins) cultures. Seven proteins were consistently regulated by quorum sensing in pure and dual cultures. M. guilliermondii proteins influenced by QS activity were also evaluated. Several up- and down- regulated proteins differed depending on the bacterial QS. These results show the QS regulation in the bacteria-yeast interactions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ygeno.2021.11.017 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antibacterial mode of action of thyme white (Thymus vulgaris L.) essential oil and its constituents, thymol and carvacrol against Agrobacterium tumefaciens via down-regulation of manganese transport genes, sitABCD and mntH.
Pestic Biochem Physiol
November 2025
Department of Agriculture, Forestry and Bioresources, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of
In this study, we evaluated the antibacterial activities of plant essential oils (EOs) from the Lamiaceae family against Agrobacterium tumefaciens to find new eco-friendly antimicrobials. Thymus vulgaris L. (thyme white) EO demonstrated the most potent fumigant antibacterial activity among these.
View Article and Find Full Text PDFUltrastructural Examination of the Fungus-To-Fungus Interactions of and .
Plant Environ Interact
October 2025
Discipline of Plant Pathology, College of Agriculture, Engineering & Science; School of Agricultural, Earth and Environmental Sciences University of KwaZulu-Natal Pietermaritzburg Republic of South Africa.
Asian soybean rust (ASR) is caused by the biotrophic fungus Syd. & P. Syd.
View Article and Find Full Text PDFEstablishment of a CRISPR/Cas9 gene editing system based on growth points transformation method in Fraxinus mandshurica.
BMC Plant Biol
August 2025
College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
The lack of an effective gene editing technology system for Fraxinus mandshurica makes it challenging to improve its traits through genetic engineering methods. In this study, an effective CRISPR/Cas9 gene editing system targeting plant growth points was established through the optimization of Agrobacterium tumefaciens concentration and infection duration. Furthermore, a tissue culture system for clustered buds was developed by supplementing the media with hormones at different concentrations.
View Article and Find Full Text PDFA Simple RUBY Reporter System in Nicotiana benthamiana for Studying Inositol Pyrophosphate Dynamics.
Methods Mol Biol
August 2025
Department of Plant Nutrition, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany.
In recent years, there has been substantial progress in the development of methods to analyze inositol phosphates (InsPs) and inositol pyrophosphates (PP-InsPs). However, many of these techniques are labor- and cost-intensive and can usually only be carried out by laboratories specialized in InsPs/PP-InsPs analysis. In this chapter, we present a simple method that exploits the fact that phosphorylation and/or dephosphorylation of certain InsP/PP-InsP species induces the activation of promoters driving the expression of genes involved in phosphate starvation response (PSR).
View Article and Find Full Text PDFMicrobial profile of the appendix niche in acute appendicitis: a novel sampling approach.
FEBS Open Bio
August 2025
The Second Hospital & Clinical Medical School, Lanzhou University, China.
Relatively little is known about the microbial variations within the human appendix niche. To overcome this knowledge gap, we employed endoscopic retrograde appendicitis treatment (ERAT) technology to collect microbial samples from the appendix lumen, followed by shotgun metagenomic sequencing on participants with acute appendicitis without antibiotic treatment. Compared to the cecum and terminal ileum, the appendix had a higher abundance at the genus level of Sphingobium, Leptotrichia and Oribacterium, as well as a significant increase in species-level abundance of oral bacteria, including Streptococcus sanguinis, Streptococcus australis, Streptococcus sp.
View Article and Find Full Text PDF