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Article Abstract
Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in , rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525380 | PMC |
| http://dx.doi.org/10.3389/fgeed.2021.617553 | DOI Listing |
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