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Article Abstract
Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO has been focused on as a green strategy to convert CO into valuable products such as fatty acids. In this study, a CO utilization pathway involving carbonic anhydrase and biotin carboxylase was formed to recycle CO in the oleaginous yeast , thereby increasing the production of fatty acids. In the recombinant strain in which the CO utilization pathway was introduced, the production of fatty acids was 10.7 g/L, which was 1.5-fold higher than that of the wild-type strain. The resulting strain had a 1.4-fold increase in dry cell mass compared to the wild-type strain. In addition, linoleic acid was 47.7% in the fatty acid composition of the final strain, which was increased by 11.6% compared to the wild-type strain. These results can be applied as an essential technology for developing efficient and eco-friendly processes by directly utilizing CO.
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| http://dx.doi.org/10.1021/acs.jafc.1c04308 | DOI Listing |
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