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A comprehensive numerical study of three-dimensional surface instability patterns is presented. The formation of wrinkles is a consequence of deformation instability when a thin film, bonded to a compliant substrate, is subject to in-plane compressive loading. We apply a recently developed computational approach to directly simulate complex surface wrinkling from pre-instability to post-instability in a straightforward manner, covering the entire biaxial loading spectrum from pure uniaxial to pure equi-biaxial compression. The simulations use embedded imperfections with perturbed material properties at the film-substrate interface. This approach not only triggers the first bifurcation mode but also activates subsequent post-buckling states, thus capable of predicting the temporal evolution of wrinkle patterns in one simulation run. The state of biaxiality is found to influence the surface pattern significantly, and each bifurcation mode can be traced back to certain abrupt changes in the overall load-displacement response. Our systematic study reveals how the loading condition dictates the formation of various instability modes including one-dimensional (1D) sinusoidal wrinkles, herringbone, labyrinth, and checkerboard.
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http://dx.doi.org/10.1038/s41598-021-95414-8 | DOI Listing |
Odontology
September 2025
Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON, Canada.
This study aimed to investigate the age-related alterations in mesial roots of mandibular first molar in terms of root canal curvature values, dentin thickness, interorifice distance, deviation from apical foramen, and location of apical foramen using a three-dimensional curvature measurement method and micro-computed tomography (micro-CT). Forty-five mesial roots of mandibular first molars from three age groups (Group 1: ≤ 30 years, Group 2: 31-59 years, Group 3: ≥ 60 years) were scanned using micro-CT. The central axis of each mesiobuccal and mesiolingual canal was analyzed using cubic B-spline curves to calculate canal curvature.
View Article and Find Full Text PDFJ Thromb Thrombolysis
September 2025
Central Laboratory of Yongchuan Hospital, Chongqing Medical University, No. 439, Xuanhua Road, Yongchuan District, Chongqing, 402160, China.
In vitro assessment of the inhibitory effect of antiplatelet drugs on platelet aggregation is frequently employed to guide personalized antiplatelet therapy in clinical practice. However, existing methods for detecting platelet aggregation rely heavily on high concentrations of exogenous agonists, which may obscure part of the inhibitory effect of antiplatelet drugs and lead to an underestimation of their effects. This study validates a novel analytical strategy for evaluating the effects of antiplatelet drugs by quantifying the microscopic three-dimensional morphological parameters of platelet aggregates formed through spontaneous aggregation on a glass surface.
View Article and Find Full Text PDFBiomater Sci
September 2025
Biotechnology Science and Engineering Program, University of Alabama in Huntsville, Huntsville, AL 35899, USA.
B cells are critical components of the adaptive immune system that proliferate and differentiate within the secondary lymphoid organs upon recognition of antigens and engagement of T cells. Traditional two-dimensional (2D) cell cultures fall short of replicating the intricate structures and dynamic evolution of three-dimensional (3D) environments found in lymphoid organs, prompting the development of more physiologically pertinent models. Our approach employs -hexanoyl glycol chitosan (HGC) coated ultra-low attachment (ULA) lattice plates to cultivate a 3D co-culture of CD40L-expressing MS5 stromal cells and naïve B cells derived from the peripheral blood mononuclear cells (PBMCs) of healthy human donors.
View Article and Find Full Text PDFLangmuir
September 2025
College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, PR China.
Sodium-ion batteries are promising candidates for large-scale energy storage due to their low cost and resource abundance. However, their cathode materials suffer from poor conductivity and limited cycling stability. Here, we report a Prussian blue (PB)-based cathode hybridized with carboxyl-functionalized carbon nanotubes (CNTs) via a glutamic acid-assisted in situ coordination route.
View Article and Find Full Text PDFJ Vis Exp
August 2025
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology.
We present multimodal confocal Raman micro-spectroscopy (RS) and tomographic phase microscopy (TPM) for quick morpho-chemical phenotyping of human breast cancer cells (MDA-MB-231). Leveraging the non-perturbative nature of these advanced microscopy techniques, we captured detailed morpho-molecular data from living, label-free cells in their native physiological environment. Human bias-free data processing pipelines were developed to analyze hyperspectral Raman images (spanning Raman modes from 600 cm to 1800 cm, which uniquely characterize a wide range of molecular bonds and subcellular structures), as well as morphological data from three-dimensional refractive index tomograms (providing measurements of cell volume, surface area, footprint, and sphericity at nanometer resolution, alongside dry mass and density).
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