Characterization of isolates of members of the genus from : description of sp. nov., sp. nov., and sp. nov.

Six novel strains (ZJ34, ZJ561, ZJ750, ZJ1629, zg-993 and zg-987) isolated from faeces and respiratory tracts of from the Qinghai-Tibet Plateau of PR China were characterized comprehensively. The results of analyses of the 16S rRNA gene and genome sequences indicated that the six strains represent three novel species of the genus , and are closely related to DSM 15434 (16S rRNA gene sequences similarities, 94.9-98.7 %), CCUG 61299 (95.6-96.6 %), CCTCC AB2010168 (95.7 %) and DSM 15435 (95.2-96.4 %), with values of digital DNA-DNA hybridization less than 30.1 % when compared with their closest relatives but higher than 70 % within each pair of novel strains (ZJ34/ZJ561, ZJ750/ZJ1629 and zg-993/zg-987). All the novel strains had C 9 and C as the two most abundant major fatty acids. MK-9(H) or MK-8(H) was the sole or predominant respiratory quinone of strains ZJ34, ZJ750 and zg-993 and their polar lipid profiles differed, but all had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and phosphatidyl inositol mannoside as major components. ZJ750 shared identical peptidoglycan amino acid profile with ZJ34 (alanine, glutamic acid, lysine and ornithine) and the same whole-cell sugar composition with zg-993 (glucose, rhamnose and ribose). Strain zg-993 contained alanine, aspartic acid, glutamic acid, glycine and lysine in the peptidoglycan, and the only sugar in ZJ34 was ribose. The DNA G+C contents of the novel strains were within the range of 65.8-70.1 mol%. On the basis of the results from the aforementioned analyses, the six novel strains were classified as representing three novel species of genus , for which the names sp. nov. [type strain ZJ34 (=GDMCC 1.1952=JCM 34355)] sp. nov. [type strain ZJ750 (=GDMCC 1.1950=JCM 34356)] and sp. nov. [type strain zg-993 (=GDMCC 1.1956=JCM 34357)] were proposed, respectively.