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SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages ("mouthwashes"). This method detects individuals with large viral loads (Ct≤29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774962 | PMC |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0244824 | PLOS |
Hum Reprod
September 2025
Institut National de la Santé et de la Recherche Médicale, Ecole des Hautes Etudes en Santé Publique, Institut de recherche en santé, environnement et travail, Université de Rennes-UMR_S1085, Rennes, France.
Study Question: What is the direct effect of mumps virus (MuV) replication within the human testis on the tissue innate immune responses and testicular cell functions?
Summary Answer: MuV induces an early pro-inflammatory response in the human testis ex vivo and infects both Leydig cells and Sertoli cells, which drastically alters testosterone and inhibin B production.
What Is Known Already: Despite widespread vaccination efforts, orchitis remains a significant complication of MuV infection, especially in young men, which potentially results in infertility in up to 87% of patients with bilateral orchitis. Our understanding of MuV pathogenesis in the human testis has been limited by the lack of relevant animal models, impairing the development of effective treatments.
Front Pharmacol
August 2025
College of Pharmacy, Binzhou Medical University, Binzhou, Shandong, China.
Introduction: Age-related macular degeneration (AMD) is a leading cause of vision loss in older adults, with limited effective treatments available. This study aimed to investigate the pharmacological effects of dihydromyricetin (DHM) on AMD and to identify its putative pharmacological targets through network analysis and molecular docking approaches.
Methods: experiments established an AMD model using sodium iodate (SI)-induced ARPE-19 cells, with CCK-8 assays determining 15 mM SI as the optimal modeling concentration and 100 μM DHM as the optimal treatment concentration.
Biochem Biophys Res Commun
August 2025
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, Yunnan Province, China. Electronic address:
Background: H1N1 influenza virus can cause diffuse alveolar damage, such as pneumonia and pulmonary fibrosis, when it infects the respiratory tract. Metformin not only improves chronic inflammation but also has direct anti-inflammatory effects. Therefore, the focus of this study was on the molecular mechanism and regulatory mechanism of metformin against influenza virus in alleviating lung disease.
View Article and Find Full Text PDFJ Cell Commun Signal
September 2025
Department of Anatomy School of Basic Medical Sciences, Qiqihar Medical University Qiqihar China.
Ductal carcinoma in situ (DCIS) is a noninvasive precursor of breast cancer with a high potential for progression. Aberrant DNA methylation plays a pivotal role in early tumorigenesis, yet the regulatory mechanisms remain incompletely defined. Integrated bioinformatic analysis of methylation and transcriptomic datasets identified miR-217 as a candidate regulator of DNA methyltransferase 1 (DNMT1).
View Article and Find Full Text PDFJ Transl Med
September 2025
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.
Fatigue is a common but poorly understood issue in type 2 diabetes (T2DM) that affects quality of life. Although ceRNA networks regulate disease progression, their role in T2DM-related fatigue (F-T2DM) is unclear. This study developed a circRNA-mediated ceRNA network to uncover the molecular interactions causing fatigue in F-T2DM.
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