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Article Abstract
Strong loss-of-function or null mutants can sometimes lead to a penetrant early lethality, impairing the study of these genes' function. This is the case for the null mutant, which exhibits 100% penetrant lethality. Here, we describe how we used gene bashing to identify distinct regulatory regions in the locus. This allowed us to generate a null strain that is viable and still displays mutant Y-to-PDA transdifferentiation phenotype. Such strategy can be applied to many other mutants impacting viability.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753896 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.000339 | DOI Listing |
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