Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
It is known that interfaces have various impacts on crystallization from a solution. Here, we describe crystallization of acetaminophen using a microflow channel, in which two liquids meet and form a liquid-liquid interface due to laminar flow, resulting in uniform mixing of solvents on the molecular scale. In the anti-solvent method, the microflow mixing promoted the crystallization more than bulk mixing. Furthermore, increased flow rate encouraged crystal formation, and a metastable form appeared under a certain flow condition. This means that interface management by the microchannel could be a beneficial tool for crystallization and polymorph control.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481770 | PMC |
| http://dx.doi.org/10.1002/elsc.202000021 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High-Performance Proteomics Using Nano-, Capillary-, and Microflow Chromatographic Separations.
J Proteome Res
September 2025
School of Life Sciences, Technical University of Munich, Emil Erlenmeyer Forum 5, Freising 85354, Germany.
Current applications of mass-spectrometry-based proteomics range from single-cell to body fluid analysis, each presenting very different demands regarding sensitivity or sample throughput. Additionally, the vast molecular complexity of proteomes and the massive dynamic range of protein concentrations in these biological systems require highly performant chromatographic separations in tandem with the high speed and sensitivity afforded by modern mass spectrometers. In this study, we focused on the chromatographic aspect and, more specifically, systematically evaluated proteome analysis performance across a wide range of chromatographic flow rates (0.
View Article and Find Full Text PDFThe role of microflow patterns combined with greyscale ultrasound in enhancing diagnostic validity and reducing unnecessary biopsy rate of thyroid nodules.
Eur Radiol
September 2025
Department of Ultrasound, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Objective: To explore the value of microflow patterns based on superb microvascular imaging (SMI) combined with greyscale ultrasound in thyroid nodule diagnosis and biopsy recommendation.
Materials And Methods: Adult patients with thyroid nodules were recruited from May 2023 to February 2024. The greyscale features of nodules were evaluated according to the five ultrasound risk stratification systems (RSSs).
An open source for multiplexed, stable and transient flows to advance life sciences using microfluidic control automation.
Lab Chip
September 2025
Institute for Life and Medical Sciences, Kyoto University, Japan.
Multiplexed fluid control is a demanding task in various studies in life sciences and bioengineering. Herein, we present open-source microfluidic sequence automation (MiSA) that offers flexible and multiplexed fluid control for various applications, providing constant flow pressure-based feedback control with 10-plex capability and pulsed flow on the order of 100 ms. MiSA was self-contained, including a pressure source, and employed an Arduino Micro to integrate ten solenoid valves, an off-the-shelf pressure regulator, and a flow sensor to balance cost and reliability.
View Article and Find Full Text PDFMicroflow LC-MS/MS reveals platform-specific post-translational modification signatures in recombinant adeno-associated virus capsids linked to enhanced potency and stability: A quality control framework for gene therapy.
J Pharm Biomed Anal
December 2025
NHC Key Laboratory of Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Beijing 100050, China. Electronic address:
Recombinant adeno-associated viruses (rAAVs) are pivotal gene therapy vectors due to their safety and stable transduction, yet comprehensive characterization of capsid post-translational modifications (PTMs)-critical for potency, immunogenicity, and manufacturing consistency-remains limited across production platforms. This study employs microflow LC-MS/MS coupled with electron-activated dissociation (EAD) to analyze PTMs in clinically relevant rAAV5 and rAAV9 serotypes produced via mammalian (HEK293) and insect (Sf9) cells, with parallel cellular-level evaluation of vector potency and infectivity, conducted under matched purity and capsid thermal stability conditions to isolate PTM-specific effects. Intact mass analysis revealed conserved N-terminal acetylation in VP1/VP3 across both platforms, while PTM profiling identified six distinct modification types, including deamidation, oxidation, and phosphorylation, with Sf9-derived vectors exhibiting 14 % more PTMs than HEK293-produced counterparts.
View Article and Find Full Text PDFToward a role for the acoustic field in cells interaction.
Front Syst Neurosci
June 2025
Institute of Matter Structure, Italian National Research Council, ISM-CNR, Rome, Italy.
Nanoscale motility of cells is a fundamental phenomenon, closely associated with biological status and response to environmental solicitations, whose investigation has disclosed new perspectives for the comprehension of cell behavior and fate. To investigate intracellular interactions, we designed an experiment to monitor movements of clusters of neuroblastoma cells (SH-SY5Y) growing on a nanomechanical oscillator (nanomotion sensor) suspended few hundreds of microns over the surface of a Petri dish where other neuroblastoma cells are freely moving. We observed that the free-to-move cells feel the presence of cells on the nearby nanosensor (at a distance of up to 300 microns) and migrate toward them, even in presence of environmental hampering factors, such as medium microflows.
View Article and Find Full Text PDF