High-speed squeeze: Light-sheet imaging of zebrafish neutrophils.

J Leukoc Biol

Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.

Published: August 2020


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Discussion of lattice light sheet microscopy used for high resolution 3D imaging of neutrophil behaviors in zebrafish larvae.

Download full-text PDF

Source
http://dx.doi.org/10.1002/JLB.1CE0320-082DOI Listing

Publication Analysis

Top Keywords

high-speed squeeze
4
squeeze light-sheet
4
light-sheet imaging
4
imaging zebrafish
4
zebrafish neutrophils
4
neutrophils discussion
4
discussion lattice
4
lattice light
4
light sheet
4
sheet microscopy
4

Similar Publications

Deep-sea polymetallic nodules, abundant in critical metal elements, are a vital strategic mineral resource. Accordingly, the prompt, accurate, and high-speed acquisition of parameters and distribution data for these nodules is crucial for the effective exploration, evaluation, and identification of valuable deposits. Studies show that one of the primary parameters for assessing polymetallic nodules is the Coverage Rate.

View Article and Find Full Text PDF

This study examines the impact of cluster jet grouting pile construction on adjacent railway lines and the control of pile body deviation through the use of stress relief holes (SF), which is critical for ensuring the safe operation of high-speed railways. Based on a construction project involving a new sewage well reinforced by jet grouting piles at a specific station, the study investigates the disturbance effects of regional cluster jet grouting pile construction on surrounding soil and the adjacent high-speed railway line, controlling variables such as the number of jet grouting piles and injection pressure. Test results indicated that the soil squeezing influence distance for a single pile is approximately 7.

View Article and Find Full Text PDF

Objective: Much of what is known about semi-occluded vocal tract (SOVT) exercise is based on modeling and theoretical constructs. This exploratory study aimed to address the need for direct kinematic data from laryngeal imaging to investigate the impact of SOVT exercise on the vibratory characteristics of the vocal folds.

Method: A small sample of two participants, with no history of voice disorder, underwent transnasal, flexible fiberoptic endoscopy connected to a custom-built high-speed videoendoscopy (HSV) system.

View Article and Find Full Text PDF

Squeezed dual-comb spectroscopy.

Science

February 2025

Department of Electrical, Computer and Energy Engineering, University of Colorado Boulder, Boulder, CO, USA.

Optical frequency combs have enabled distinct advantages in broadband, high-resolution spectroscopy and precision interferometry. However, quantum mechanics ultimately limits the metrological precision achievable with laser frequency combs. Quantum squeezing has led to substantial measurement improvements with continuous wave lasers, but experiments demonstrating metrological advantage with squeezed combs are less developed.

View Article and Find Full Text PDF

Nanoscale dynamics of Dynamin 1 helices reveals squeeze-twist deformation mode critical for membrane fission.

Proc Natl Acad Sci U S A

December 2024

Materials Science Division, Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550.

Dynamin 1 (Dyn1) GTPase, a principal driver of membrane fission during synaptic endocytosis, self-assembles into short mechanoactive helices cleaving the necks of endocytic vesicles. While structural information about Dyn1 helix is abundant, little is known about the nanoscale dynamics of the helical scaffolding at the moment of fission, complicating mechanistic understanding of Dyn1 action. To address the role of the helix dynamics in fission, we used High-Speed Atomic Force Microscopy (HS-AFM) and fluorescence microscopy to track and compare the spatiotemporal characteristics of the helices formed by wild-type Dyn1 and its K44A mutant impaired in GTP hydrolysis on minimal lipid membrane templates.

View Article and Find Full Text PDF