Confers Drought Stress Tolerance in Transgenic Rice by Regulating Lignin Biosynthesis and ABA Signaling Pathway.

Front Plant Sci

National Key Facility for Crop Genetic Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Published: June 2020


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Article Abstract

Foxtail millet () originated in China and is generally cultivated in arid and barren soil. Through long-term harsh environmental selection, foxtail millet has acquired significant drought resistance. However, the molecular mechanism of foxtail millet drought resistance is still unknown. Here, we identified a drought-induced R2R3-MYB transcription factor in foxtail millet. Overexpression of significantly enhances tolerance to drought stress in transgenic rice plants at both the vegetative and the reproductive stage and has no adverse effect on its normal growth. Compared with wild-type controls, -overexpressing rice plants had lower MDA content and higher lignin content under drought conditions. Quantitative real-time PCR and Transcriptional activity assays demonstrated that could activate expression of lignin biosynthesis genes under drought conditions. Also, we found that overexpression of can led to ABA accumulation in the seeds transgenic rice plants. Further experiments showed that Overexpression of can upregulate the expression of ABA synthesis and response related genes under drought conditions. In conclusion, may enhance the drought resistance of transgenic rice plants by regulating lignin biosynthesis and ABA signaling pathway, making a candidate gene for drought resistance improvement in gramineous crops.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314972PMC
http://dx.doi.org/10.3389/fpls.2020.00785DOI Listing

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