Upregulation of long noncoding RNA promotes tumor metastasis and predicts poor prognosis in lung cancer.

Background: Lung cancer (LC) is one of the leading causes of cancer-related mortality in China and worldwide. Despite the progress in diagnosis and treatment of LC, the prognosis of LC remains poor. Studies have demonstrated that long non-coding RNAs (lncRNAs) play a critical role in carcinogenesis and cancer development.

Methods: Here we examined the expression and potential function of in LC both and . All experiments in this study were conducted using A549 and PC-9 cell lines according to protocols described in this paper. The clinic characteristics were analyzed using logistic regression, cox model, log rank test, biochemical analysis using qRT-PCR, transfections, nude mice model, and cell biological analysis using Transwell assay, CCK-8 assay, flow cytometry, and rescue experiments, and immunohistochemistry.

Results: The results showed that was significantly overexpressed in LC tissues compared to the corresponding non-tumor tissues. Patients with a higher level of expression showed a poorer overall survival rate. Functionally, overexpression of promotes cell proliferation, migration and invasion abilities of LC cell lines, which suggests may play an oncogenic role in LC. was located in physical contiguity with gene and found positively regulates the expression, and the protein levels of RAB11B in LC tissues also found to positively correlated with the level of expression. silencing partially abrogated -induced proliferation of the LC cell lines used in this study.

Conclusions: This study provided a novel evidence into the function of lncRNA-driven carcinogenesis. Our findings highlighted the importance of and in LC progression and indicated that may serve as a novel and valuable prognostic biomarker for LC.