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Traditionally the focus for control of food-borne disease has been bacteria. During the last decade viruses have emerged as important sources of food borne human disease. Since the traditional bacteriological indicators, are not reliable for viral contamination, new methods are needed. PCR has enhanced the detection of virus in food. A challenge for developing detection reliable methods for viruses in food is that food matrices vary in composition, high sequence variability and inhibitors may be present. Therefore it is necessary to develop assays that have high diagnostic sensitivity, are broad and robust, and combine sample concentration and removal of inhibitors.
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http://dx.doi.org/10.1016/j.tifs.2011.05.008 | DOI Listing |
Indoor Air
January 2025
National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Background/objectives: Respiratory viruses circulate year-round and can spread indoors via inhalation of airborne particles. Effective ventilation and filtration may reduce transmission, particularly in school settings where children and staff spend significant time. This study examines the impact of indoor air quality (IAQ) and ventilation in schools on respiratory virus detection.
View Article and Find Full Text PDFCan Vet J
September 2025
Alberta Beef Health Solutions, PO Box 307, Picture Butte, Alberta T0K 1V0 CANADA (Brookhart, Dimmers); Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta T3R 1J3 CANADA (Goldsmith, Janzen, Olchowy, Morrison, Zachar, Remnant. Rogers, Waine); Veterinary Biomedical Sciences, Wester
Over 1200 heavy feedlot cattle presented with severe tongue lesions during a 7-month interval. Review of the literature and discussions with colleagues revealed previous similar outbreaks had occurred in Canada and the USA, with extensive investigations conducted and no cause identified. In the current outbreak, examination of the environment, feed, water, cattle behavior, and husbandry systems were conducted, as well as diagnostic testing including necropsy, histopathology, bacteriology, virology, and toxicology.
View Article and Find Full Text PDFJ Med Internet Res
September 2025
Artificial Intelligence and Mathematical Modeling Lab, Dalla Lana School of Public Health, University of Toronto, Toronto, ON, Canada.
Background: The H5N1 avian influenza A virus represents a serious threat to both animal and human health, with the potential to escalate into a global pandemic. Effective monitoring of social media during H5N1 avian influenza outbreaks could potentially offer critical insights to guide public health strategies. Social media platforms like Reddit, with their diverse and region-specific communities, provide a rich source of data that can reveal collective attitudes, concerns, and behavioral trends in real time.
View Article and Find Full Text PDFJAMA Netw Open
September 2025
Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia.
Importance: Long COVID (ie, post-COVID-19 condition) is a substantial public health concern, and its association with health-related social needs, such as food insecurity, remains poorly understood. Identifying modifiable risk factors like food insecurity and interventions like food assistance programs is critical for reducing the health burden of long COVID.
Objective: To investigate the association of food insecurity with long COVID and to assess the modifying factors of Supplemental Nutrition Assistance Program (SNAP) participation and employment status.
Nucleic Acids Res
September 2025
School of Microbiology, University College Cork, Cork, T12 Y337, Ireland.
The genomes of 43 distinct lactococcal strains were reconstructed by a combination of long- and short-read sequencing, resolving the plasmid complement and methylome of these strains. The genomes comprised 43 chromosomes of approximately 2.5 Mb each and 269 plasmids ranging from 2 to 211 kb (at an average occurrence of 6 per strain).
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