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Polyhydroxyalkanoates (PHAs) are inevitably a key biopolymer that has the potential to replace the conventional petrochemical based plastics that pose jeopardy to the environment globally. Even then the reach of PHA in the common market is so restricted. The economy of PHA is such that, even after several attempts the overall production cost seems to be high and this very factor surpasses PHAs usage when compared to the conventional polymers. The major focus of the review relies on the synthesis of PHA from Mixed Microbial Cultures (MMCs), through a 3-stage process most probably utilizing feedstocks from waste streams or models that mimic them. Emphasis was given to the works carried out in the past decade and their coherence with each and every individual criteria (Aeration, Substrate and bioprocess parameters) such that to understand their effect in enhancing the overall production of PHA.
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http://dx.doi.org/10.1016/j.biortech.2020.123132 | DOI Listing |
FEBS Open Bio
September 2025
Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
The global accumulation of plastic waste, exceeding 360 million tonnes annually, represents a critical environmental challenge due to their widespread use and extreme recalcitrance in natural environments. Furthermore, the end-of-life processing of bioplastics, which are often marketed as eco-friendly, remains problematic, with biodegradation often requiring industrial conditions. Enzyme-based depolymerization of polyesters, such as polyethylene terephthalate (PET) and bioplastics (e.
View Article and Find Full Text PDFPrep Biochem Biotechnol
September 2025
Department of Biotechnology, Manipal Institute of Technology Manipal Academy of Higher Education, Manipal, Udupi, India.
Polyhydroxyalkanoates (PHAs), biodegradable biopolyesters produced by bacteria, offer an eco-friendly alternative to synthetic plastics. However, current methods of recovering intracellular PHA require complex cell lysis steps, which contribute to high production costs. In this study, we developed a novel approach for efficient PHA recovery by engineering an autolytic strain of using the lytic gene system from lambda (λ) phage.
View Article and Find Full Text PDFBioresour Bioprocess
August 2025
Amity Institute of Microbial Technology, Amity University, Noida, 201313, India.
Plastics are essential components of modern life, and their global demand is increasing daily. They are gaining recognition as a sustainable source for bioplastic production due to their rapid growth, carbon fixation ability, and capacity to utilize various waste streams. It seems that landfill, incineration, chemical treatment, and plastic recycling are not the best options for minimizing plastic pollution.
View Article and Find Full Text PDFMacromol Biosci
August 2025
Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Poly[(R)-3-hydroxybutyrare-co-(R)-3-hydroxypivalate] (P(3HB-co-3HPi)) films, a type of polyhydroxyalkanoate (PHA), are oxidized using photoactivated chlorine dioxide radical (ClO•) gas to generate carboxyl groups and loaded with divalent metal cations, including Cu, Zn, and Ca ions, via ionic interactions. The P(3HB-co-3HPi) films loaded with Cu ions exhibit enhanced antibacterial activity against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) compared with untreated P(3HB-co-3HPi) films. In seawater, the biodegradation of these Cu and Zn-loaded films is initially inhibited by the antimicrobial activity of the cations and occurs gradually; therefore, loading antimicrobial divalent metal cations onto the surface of PHAs inhibits biodegradation in seawater temporarily but allows biodegradation to occur with time.
View Article and Find Full Text PDFACS Omega
August 2025
Department of Mechanical Engineering, Hellenic Mediterranean University, Heraklion 71410, Greece.
Polyhydroxyalkanoate (PHA) is a biopolymer that can be 3D printed using the material extrusion method. Nevertheless, their mechanical properties are inferior to those of petroleum-derived polymers, which restricts their broader application. Herein, nanobiocomposites comprising naturally sourced PHA and cellulose nanocrystals (CNC) as fillers were successfully synthesized.
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