Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Objectives: The aim of this study was to analyse the protein profiles of acquired enamel pellicle (AEP) at two time points (5 min and 2 h) between caries-susceptible and caries-free subjects using label-free and parallel reaction monitoring (PRM) proteomic approaches, and to discover potential biomarkers for dental caries.
Methods: Sixty participants (30 caries-susceptible (DMFT>5) and 30 caries-free subjects (DMFT = 0)) were included. Their AEP at 5 min and 2 h was separately sampled, and the AEP materials were quantitatively analysed using label-free proteomics. Bioinformatics analysis of differentially expressed proteins was subsequently conducted and target proteins were verified using PRM analysis.
Results: A total of 82 and 39 differentially expressed proteins were identified at the 5-minute and 2 -h points, respectively, between the caries-susceptible and caries-free groups. Some proteins related to immune response and antibacterial activity were identified, and the characteristic enriched biological processes of up- and down-regulated proteins were presented using bioinformatics analysis. Subsequently, five intriguing target proteins of each group were chosen for PRM analysis and their presence was successfully verified in the 5-minute and 2 -h AEPs, respectively.
Conclusions: The proteins in the AEPs of caries-susceptible and caries-free subjects at both time points presented unique protein profiles related to immune response and antibacterial activity. Mucin-7 can be regarded as an important potential AEP biomarker for dental caries.
Clinical Significance: The results of the study identified potential biomarkers, which can facilitate the design of new bio-functional agents for the diagnosis and treatment of dental caries in future.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jdent.2020.103301 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessment of Vitamin E and Glutathione Peroxidase Levels in Salivary Samples of Children With and Without Dental Caries in Erbil City, Iraq.
Cureus
November 2024
Dental Public Health, Kurdistan Higher Council of Medical Specialties, Erbil, IRQ.
Introduction: According to the classic understanding of the etiology of dental caries, an imbalance between demineralization and remineralization in the oral cavity is important. Salivary antioxidants, including glutathione peroxidase and vitamin E, can modulate oxidative stress.
Methods: This cross-sectional study evaluated the levels of glutathione peroxidase and vitamin E in the saliva of 90 children from Erbil City.
Deep Saliva Proteomics Elucidating the Pathogenesis of Early Childhood Caries and Identifying Biomarkers for Early Prediction.
J Proteome Res
February 2025
Department of Pediatric Dentistry, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomato
Article Synopsis
- Early childhood caries (ECC) greatly affects children's health, and saliva is essential for studying its causes due to its reflection of both the body and microbes.
- Researchers followed a group of 3-4 year-olds for a year, using advanced proteomics to analyze saliva and identify mechanisms behind caries development and potential biomarkers.
- The study found specific salivary proteins linked to caries susceptibility, created a prediction panel (KRT3 and MUC21), and highlighted the importance of saliva in preventing ECC, aiming to reduce its occurrence among children.
Comparative evaluation of occlusal pits and fissures morphology modification techniques before application of sealants: An study.
Indian J Dent Res
May 2020
Department of Dentistry, Sri Shankaracharya Institute of Medical Sciences, Bhilai, Durg, Chhattisgarh, India.
Background: Pits and Fissures are recognized as being highly susceptible to caries. Pit and fissure sealants are one of the best methods of preventing caries as it occludes the fissures and pits from the accumulation of plaque and cariogenic microflora. There are different methods of cleaning and preparing occlusal pits and fissures for preventing caries which helps in alleviating oral health status of paediatric population.
View Article and Find Full Text PDFComparative proteomic analysis on acquired enamel pellicle at two time points in caries-susceptible and caries-free subjects.
J Dent
March 2020
State Key Laboratory of Oral Diseases and National Clinical Research Centre for Oral Diseases, Sichuan University, No.14, Section 3 of Renmin Road South, Chengdu, 610041, China; Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, No.14, Section 3 of Renmi
Objectives: The aim of this study was to analyse the protein profiles of acquired enamel pellicle (AEP) at two time points (5 min and 2 h) between caries-susceptible and caries-free subjects using label-free and parallel reaction monitoring (PRM) proteomic approaches, and to discover potential biomarkers for dental caries.
Methods: Sixty participants (30 caries-susceptible (DMFT>5) and 30 caries-free subjects (DMFT = 0)) were included. Their AEP at 5 min and 2 h was separately sampled, and the AEP materials were quantitatively analysed using label-free proteomics.
iTRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals.
J Transl Med
October 2018
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Cariology and Endodontics West China Hospital of Stomatology, Sichuan University, No. 14, Section 3 of Renmin South Road, Chengdu, Sichuan, China.
Background: Human saliva is a protein-rich, easily accessible source of potential biomarkers for the diagnosis of oral and systemic diseases. However, little is known about the changes in salivary proteome associated with aging of patients with dental caries. Here, we applied isobaric tags for relative and absolute quantitation (iTRAQ) in combination with multiple reaction monitoring mass spectrometry (MRM-MS) to characterize the salivary proteome profiles of subjects of different ages, presenting with and without caries, with the aim of identifying age-related biomarkers for dental caries.
View Article and Find Full Text PDF