Techno-economic analysis for extracellular-polymeric substances (EPS) production using activated sludge fortified with crude glycerol as substrate and its application in leachate treatment.

Economic assessment of bio-flocculant production process has been carried out by SuperPro Designer where extracellular-polymeric substances (EPS) were produced using activated sludge fortified with crude glycerol in fermenter followed by centrifugation. Considering EPS concentration of 60 g/L in production fermenter at 96 h, the unit production cost for slime EPS was estimated to be $ 0.95/L. The unit price of S-EPS was sensitive to inoculum size and EPS productivity (EPS concentration and fermentation time) in the fermented broth. Economic analysis was also conducted for EPS aided leachate treatment. The unit leachate treatment cost was 7.78 $/m and was sensitive to S-EPS unit production cost. To get same leachate treatment cost as current industrial practice (4 $/m), S-EPS unit production cost should lower down to $ 0.5/L. The process has several advantages: 1) sludge and crude glycerol valorization for bio-flocculant production 2) Leachate treatment using environment friendly bio-flocculant.