Metabolite profiling and wound-healing activity of leaf extracts using and models.

is a perennial herb belonging to the Nyctaginaceae family. This plant has been widely used in Indian traditional medicinal system to cure several human ailments. However, traditional use of this plant in the treatment and management of wounds has not been validated by any comprehensive scientific study. The present study was aimed to explore the and wound healing potential of methanol extract (ME) and chloroform extract (CE) from leaf and subsequent identification of the bioactive metabolites, which might be responsible for enhancement of wound healing property of the extracts. The study included cell viability and wound scratch assays as well as excision wound assays in rat models. Both ME and CE were analysed for their antioxidant properties and phenolics content. The gas chromatography-mass spectrometry analyses were performed for identification of bioactive metabolites present in the ME and CE. ME of leaf significantly enhanced viability and migration of human keratinocyte cells (HaCaT) as compared to the untreated and CE-treated groups. The topical application of ME of leaf in excision wound model significantly decreased the wound area by the 14th day (91%) as compared to control (22%) ( < 0.05). The GC-MS analysis revealed the presence of caffeic acid, ferulic acid and D-pinitol as the major bioactive metabolites in ME. These results suggest that ME of possesses significant wound healing potential, where D-pinitol and caffeic acid served as the lead constituent metabolites responsible for the healing.