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We report a novel nanostructured chemosensing ensemble PyNp-C13/UD, obtained by self-assembling uranine dye (UD) and an amphiphilic pyridinium salt PyNp-C13. The ensemble was developed for the fluorescence turn-on sensing of ATP in aqueous solutions and inside living cells. The assembly operates via an indicator displacement assay (IDA) method with an ultra-low detection limit of 6.8 nM.
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http://dx.doi.org/10.1039/c9cc08054f | DOI Listing |
Dalton Trans
August 2025
Institute of Chemistry, National Autonomous University of Mexico, Ciudad Universitaria, México, 04510, CDMX, Mexico.
Selective recognition and sensing of neurotransmitters in aqueous media using artificial receptors is an attractive but challenging goal in modern supramolecular chemistry. Despite advances in the development of optical receptors for some neurotransmitters, such as dopamine, limited efforts have been invested in developing receptors for epinephrine, a neurotransmitter of paramount importance and a widely used drug for heart attacks. Herein, a new fluorescent molecular receptor (referred to as 1) based on a 1,3-bis-benzimidazole-benzene derivative covalently linked to two phenyl boronic acids was synthesized, structurally characterized single-crystal X-ray diffraction, and studied in-depth as a receptor for four catecholamine-based neurotransmitters as well as several nucleosides, monosaccharides and L-tyrosine in water at physiological pH.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
March 2025
School of Science, Constructor University, Campus Ring 1, 28759, Bremen, Germany.
Compartmentalized models with coupled catalytic networks are considered as "protocells" in the context of research related to the origin of life. To model the kinetics of a simple cellular uptake-metabolism process, we use a compartmentalized protocell system that combines liposome-encapsulated intravesicular reporter pairs with co-encapsulated enzymes to monitor the membrane transport of a substrate (analyte uptake) and its subsequent enzymatic reaction inside the vesicles (metabolism to the product). The intravesicular chemosensing ensembles consist of the macrocycles cucurbit[7]uril or p-sulfonatocalix[4]arene and matching fluorescent dyes to set up suitable reporter pairs.
View Article and Find Full Text PDFNanoscale
November 2023
School of Chemical Science and Engineering, Department of Clinical Laboratory, Shanghai Tenth People's Hospital, Tongji University, 1239 Siping Rd, Shanghai, 200092, PR China.
The most promising alternative for next-generation molecular computers is biocomputing, which uses DNAs as its primary building blocks to perform a Boolean operation. DNA nanoclusters (NCs) have emerged as promising candidates for biosensing applications due to their unique self-assembly properties and programmability. It has been demonstrated that adding DNA overhangs to DNA NCs improves their adaptability in identifying specific biomolecular interactions.
View Article and Find Full Text PDFAdv Mater
January 2024
School of Science, Constructor University, Campus Ring 1, 28759, Bremen, Germany.
The design of synthetic systems with interrelated reaction sequences that model incipient biological complexity is limited by physicochemical tools that allow the direct monitoring of the individual processes in real-time. To mimic a simple digestion-resorption sequence, the authors have designed compartmentalized liposomal systems that incorporate extra- and intravesicular chemosensing ensembles. The extravesicular reporter pair consists of cucurbit[7]uril and methylene blue to monitor the enzymatic cleavage of short enkephalin-related peptides by thermolysin through a switch-off fluorescence response ("digestion").
View Article and Find Full Text PDFChempluschem
May 2023
Department of Pure and Applied Sciences, University of Urbino "Carlo Bo", Via della Stazione 4, 61029, Urbino, Italy.
The tetranuclear Cu /Ca /Ca2 /Cu complex based on Malten ligand has been investigated as a platform for anion binding. Simple organic carboxylates and non-steroidal anti-inflammatory drugs (NSAIDs) have been tested, revealing the ability of the platform to bind them. The receiving platform hosts at least two guests in solution although a third anion can be bound, as suggested by X-ray diffraction analysis.
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