Isolation and Characterization of Two New Metabolites from the Sponge-Derived Fungus sp. LS34 by OSMAC Approach.

The application of an OSMAC (One Strain-Many Compounds) approach on the sponge-derived fungus sp. LS34, using two different media including solid rice medium and potato dextrose broth (PDB) resulted in the isolation and identification of two new compounds, named asperspin A () and asperther A () along with seven known compounds -. Compounds - were detected in fungal extracts from rice medium, while compounds - were isolated from PDB medium. Their structures were unambiguously characterized by HRESIMS and NMR spectroscopic data. The growth inhibitory activity of these compounds against four pathogenic bacteria (, , , and ) were evaluated. All the compounds were also tested for their cytotoxicity against seven cancer cell lines, including CCRF-CEM, K562, BGC823, AGS, HCT-116, MDA-MB-453, and COR-L23. Among them, compound showed strong activity against CCRF-CEM and K562 cells with IC values of 1.22 ± 0.05 µM and 10.58 ± 0.19 µM, respectively. Notably, compound also showed pronounced activity against with an MIC value of 3.54 µM.