98%
921
2 minutes
20
The helium ion microscope (HIM) has emerged as an instrument of choice for patterning, imaging and, more recently, analytics at the nanoscale. Here, we review secondary electron imaging on the HIM and the various methodologies and hardware components that have been developed to confer analytical capabilities to the HIM. Secondary electron-based imaging can be performed at resolutions down to 0.5 nm with high contrast, with high depth of field, and directly on insulating samples. Analytical methods include secondary electron hyperspectral imaging (SEHI), scanning transmission ion microscopy (STIM), backscattering spectrometry and, in particular, secondary ion mass spectrometry (SIMS). The SIMS system that was specifically designed for the HIM allows the detection of all elements, the differentiation between isotopes, and the detection of trace elements. It provides mass spectra, depth profiles, and 2D or 3D images with lateral resolutions down to 10 nm.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1146/annurev-anchem-061318-115457 | DOI Listing |
Electrophoresis
September 2025
Therapeutics Development and Supply-Analytical Development, Janssen Research & Development, LLC, Malvern, Pennsylvania, USA.
Monoclonal antibodies (mAbs) present analytical challenges due to their inherent heterogeneity and susceptibility to post-translational modifications (PTMs) during production and storage. Monitoring of charge heterogeneity profiles by imaged capillary isoelectric focusing (icIEF) has been aided by the use of non-detergent sulfobetaines (NDSBs), particularly NDSB-211, to enhance protein solubility and stability. When used in a quality control laboratory setting, NDSB-211 has shown performance variability over time due to residual manufacturing impurities that impact the capillary isoelectric focusing separation.
View Article and Find Full Text PDFJ Marital Fam Ther
October 2025
Department of Dynamic and Clinical Psychology, and Health Studies, Sapienza University of Rome, Rome, Italy.
This systematic review and meta-analysis examines the role of romantic attachment as a protective or risk factor in how individuals cope with infertility diagnosis, treatment, and outcomes. A systematic search was conducted across six databases from January 1, 2011, to February 3, 2025. Seventeen studies met inclusion criteria, exploring associations between romantic attachment and individual psychological correlates of infertility.
View Article and Find Full Text PDFInfect Disord Drug Targets
September 2025
Department of Chemistry, NFC Institute of Technology, Multan, Pakistan.
Introduction: Targeted infection imaging is crucial for accurate diagnosis in postpartum women. This project uses 99mTc-labeled cefixime to develop a radiopharmaceutical for detecting, distinguishing, and treating infections and abscesses in women.
Method: Technetium (TcO4-) chelated with cefixime, reduced by stannous chloride, confirmed via thin-layer chromatography.
J Dent Educ
September 2025
Department of Oral and Maxillofacial Surgery, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, P. R. China.
Background: Virtual reality (VR) and artificial intelligence (AI) technologies have advanced significantly over the past few decades, expanding into various fields, including dental education.
Purpose: To comprehensively review the application of VR and AI technologies in dentistry training, focusing on their impact on cognitive load management and skill enhancement. This study systematically summarizes the existing literature by means of a scoping review to explore the effects of the application of these technologies and to explore future directions.
Anal Chim Acta
November 2025
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China. Electronic address:
Background: The development of specific fluorescent probes for cancer cell discrimination holds significant promise for advancing cancer diagnostics. Conventionally, these probes operate by translating differences in biomarkers or microenvironmental factors into variations in whole-cell fluorescence intensity. However, this dominant, intensity-based strategy is highly susceptible to extraneous fluctuations arising from probe concentration, illumination instability and complex intracellular environment.
View Article and Find Full Text PDF