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Rhamnolipid produced from a Lysinibacillus sphaericus IITR51 was characterized and its ability for dissolution of hydrophobic pesticides were evaluated. L. sphaericus produced 1.6 g/L of an anionic biosurfactant that reduced surface tension from 72 N/m to 52 N/m with 48% emulsification index. The biosurfactant was found stable over a wide range of pH (4.0-10.0), temperature (4-100 °C), salt concentration (2-14%) and was identified as rhamnolipid. At the concentration of 90 mg/L rhamnolipid showed enhanced dissolution of α-, β-endosulfan, and γ-hexachlorocyclohexane up to 7.2, 2.9, and 1.8 folds, respectively. The bacterium utilized benzoic acid, chlorobenzene, 3- and 4-chlorobenzoic acid as sole source of carbon and was found resistant to arsenic, lead and cadmium. Furthermore, the isolated biosurfactant showed antimicrobial activities against different pathogenic bacteria. The results obtained indicate the usefulness of rhamnolipid for enhanced dissolution and thereby increasing the bioavailability.
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http://dx.doi.org/10.1016/j.biortech.2018.09.144 | DOI Listing |
Mar Genomics
August 2025
College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China. Electronic address:
Lysinibacillus sp. WB86 was isolated from a cold seep in the South China Sea, and its complete genome was sequenced using Oxford Nanopore Technologies (ONT). The genome consists of a single circular chromosome spanning 4,537,071 bp, with a G + C content of 37 %.
View Article and Find Full Text PDFWater Sci Technol
July 2025
Faculdade de Tecnologia, Universidade Estadual de Campinas (UNICAMP), Paschoal Marmo 1888, Limeira, SP 13484332, Brazil.
The main objective of this study was to characterize metagenomically and evaluate the biofilter effectiveness for COD, total coliforms, , and Helminth eggs removal from urban wastewater using different configurations. Several contact times of wastewater with the biofilm were tested, as well as physicochemical and biological parameters. The optimal treatment time for COD removal was 480 min for BAC1 and 360 min for BAC2, while for removal, it was 90 and 120 min by BAC1 and BAC2, respectively, and 200 min for Helminth eggs by both BACs.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Yossuf Abbas st., P.O. 11754, Nasr City, Cairo, Egypt.
Finding a safe and efficient broad-spectrum antiviral agent is the main focus of recent research with increasing desires for biosurfactants as therapeutic agents. This study aimed to produce and characterize biosurfactant from Lysinibacillus sphaericus and determine its antiviral activities against both Herpes simplex virus type 1 (HSV-1) and Hepatitis A virus (HAV). The initial oil displacement zone for biosurfactant screening was 8.
View Article and Find Full Text PDFInt J Environ Health Res
July 2025
School of Materials, Energy, Water and Environmental Sciences (MEWES), The Nelson Mandela African Institution of Science and Technology (NM-AIST), Arusha, Tanzania.
A systematic review of the scientific literature was conducted with the objective of finding evidence on the contribution of microbial larvicides, var and , in clinical malaria burden in Africa. A systematic literature search was carried out using databases PubMed, Scopus, Web of Science, ProQuest, and BASE, and Google, and Google Scholar search engines. All results were screened for duplicates and assessed for eligibility.
View Article and Find Full Text PDFFront Microbiol
June 2025
Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Japan.
Background: Safranin O is commonly used for the gram staining of bacteria and fluorescent staining of plant tissues. We aimed to perform a more detailed structural analysis of bacterial spores by analyzing the staining pattern of safranin O, together with a combination of other fluorescence probes, including 2-(4-aminophenyl) benzothiazole (APBT).
Methods: We stained spores from six species, including , and , with safranin O and APBT and observed them using fluorescence microscopy.