Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Fluorescent protein-based biosensors are providing us with an unprecedented, quantitative view of the dynamic nature of the cellular networks that lie at the heart of plant biology. Such bioreporters can visualize the spatial and temporal kinetics of cellular regulators such as Ca and H, plant hormones and even allow membrane transport activities to be monitored in real time in living plant cells. The fast pace of their development is making these tools increasingly sensitive and easy to use and the rapidly expanding biosensor toolkit offers great potential for new insights into a wide range of plant regulatory processes. We suggest a checklist of controls that should help avoid some of the more cryptic issues with using these bioreporter technologies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.pbi.2018.07.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of Microplastics Pollution Using a Green Fluorescent Protein-Based Microbial Biosensor Coupled with Raman Spectroscopy.
ACS Sens
September 2025
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon 999077, Hong Kong SAR China.
The persistence of plastics in the environment, especially after waste disposal, poses a significant threat to ecosystems. Microplastics (MPs) are particularly concerning due to their small size and the difficulty of detection. Once in aquatic systems, MPs threaten marine life and human health through the food chain.
View Article and Find Full Text PDFDesigner Frankenproteins That Halt the Proliferation of Myc-Driven Cancer Cells.
J Med Chem
September 2025
Department of Chemistry, University of Toronto, 3359 Mississauga Road, Mississauga, Ontario L5L 1C6, Canada.
Overexpression of the proto-oncogene MYC occurs in >70% of cancers and is especially prevalent in breast cancer. Myc partners with transcription factor Max to bind to the E-box DNA response element. By patterning our frankenproteins on the basic region/helix-loop-helix/leucine zipper motif of Max, we designed MEF and MEF/C93 to bind to the E-box.
View Article and Find Full Text PDFNano-Biosensors for mRNA-Based Cell Sorting Using Intracellular Markers at the Early Stage of Cell Reprogramming.
Adv Funct Mater
January 2025
Department of Bioengineering, University of California, Los Angeles, CA 90095, USA.
Cell reprogramming and manufacturing have broad applications in tissue regeneration and disease treatment. However, many derived cell types lack unique cell surface markers for protein-based cell sorting, making it difficult to isolate these cells from mixed populations. Additionally, there is a need to identify and isolate cells of interest at the early stages of cell expansion.
View Article and Find Full Text PDFEffects of Cassava and Modified Starch on the Structural and Functional Characteristics of Peanut Protein-Based Meat Analogs.
Foods
August 2025
College of Grain Science and Technology, Shenyang Normal University, Shenyang 110034, China.
Meat analog manufacturing via high-moisture extrusion technology is a complex process wherein the properties of protein materials constitute a critical determining factor. In this study, we enhanced the fiber structure properties of high-moisture extruded peanut protein-based meat analogs by incorporating different starches (cassava starch, acetyl distarch phosphate [ADSP], and hydroxypropyl starch) to address challenges in water retention, emulsification, and digestibility. The impact of the starch content (0, 3, 6, 9, 12%) was assessed using low-field nuclear magnetic resonance, ultraviolet/fluorescence spectroscopy, differential scanning calorimetry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and functional tests.
View Article and Find Full Text PDF[Construction of IgG4 Fc variants and their serum half-lives].
Sheng Wu Gong Cheng Xue Bao
August 2025
School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
In this study, we constructed a series of recombinant Fc variants of immunoglobulin G4 (IgG4), screened the fragment crystallizable (Fc) variants with significantly prolonged serum half-lives, and analyzed the relationship between mutation site and half-life, aiming to provide a theoretical basis for the development of IgG4 antibodies and Fc fusion protein-based drugs. Nine gene sites were selected for mutation, and different mutation sites were combined. The variant expression plasmids pET24b-Fc were constructed by molecular cloning and point mutation.
View Article and Find Full Text PDF