98%
921
2 minutes
20
Ziziphus nummularia (Burm.f.) Wight & Arn., a perennial shrub that thrives in the arid regions, is naturally tolerant to drought. However, there are limited studies on the genomics of drought tolerance in Ziziphus sp. In this study, RNA-sequencing of one month old seedlings treated with PEG 6000 was performed using Roche GS-FLX454 Titanium pyrosequencing. A total of 367,176 raw sequence reads were generated, and upon adapter trimming and quality filtration 351,872 reads were assembled de novo into 32,739 unigenes. Further characterization of the unigenes indicated that 73.25% had significant hits in the protein database. Kyoto encyclopedia of genes and genomes database (KEGG) identified 113 metabolic pathways from the obtained unigenes. A large number of drought-responsive genes were obtained and among them differential gene expression of 16 highly induced genes was validated by qRT-PCR analysis. To develop genic-markers, 3,425 simple sequence repeats (SSRs) were identified in 2,813 unigene sequences. The data generated shall serve as an important reservoir for the identification and characterization of drought stress responsive genes for development of drought tolerant crops.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799245 | PMC |
http://dx.doi.org/10.1038/s41598-018-20548-1 | DOI Listing |
Sci Rep
July 2025
Department of Biology, American University of Beirut, Beirut, 1107, Lebanon.
While inflammation is a vital immune response to harmful stimuli, chronic inflammation has been associated with several diseases such as arthritis, cardiovascular disorders, and cancers. Plant extracts, rich in bioactive compounds, have been used in traditional medicine to manage inflammation by modulating inflammatory pathways. Specifically, Ziziphus nummularia (Sidr), known for its anti-inflammatory, antioxidant, and antimicrobial properties, contains flavonoids, saponins, triterpenoids, and phenolics, but its mechanisms of action remain unclear.
View Article and Find Full Text PDFEcol Evol
May 2025
Department of Soil Science, School of Agriculture Shiraz University Shiraz Iran.
Climate change, a global threat of utmost significance, has the potential to trigger shifts in biodiversity distribution and the emergence of novel ecological communities. While considerable research has focused on predicting the impacts of climate change on the range shift of species, a critical yet often overlooked aspect is the role of changing climate on plants in hot, arid, and poorly known ecosystems. We employed an ensemble species distribution modeling framework to investigate how climate change might affect the spatial range of two significant indicator species, and , within the hot and arid Khalijo-Omanian ecosystem of Iran.
View Article and Find Full Text PDFNarra J
April 2025
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universiti Malaya, Kuala Lumpur, Malaysia.
The p53 signaling pathway plays a critical role in regulating the cell cycle, apoptosis, and senescence, making it a key target in cancer research. The aim of this study was to investigate the effects of an ethanol extract from the stem of on the proliferation and expression of genes involved in the p53 pathway in MCF-7 breast cancer cells. To achieve this, real-time quantitative PCR (RT-qPCR) was used to evaluate the mRNA expression of downstream genes linked to cell cycle and senescence, including or Molecular docking simulations using Molegro Virtual Docker (MVD) were also performed to assess the potential inhibitory activity of metabolite compounds from stem against p53-regulating kinase (TP53RK.
View Article and Find Full Text PDFScientifica (Cairo)
September 2024
Dendrochronology Lab Department of Botany Government College University, Lahore 54000, Pakistan.
The present microstructural evaluation was carried out on the woods of three ethnobotanically important local fruit trees, namely, Lam., Z. (L.
View Article and Find Full Text PDFPhysiol Plant
July 2024
Biofuels Institute, School of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu, China.
The Ziziphus genus, belonging to the Rhamnaceae family, holds significant economic, nutritional, and medicinal value. However, much remains to be discovered about its diversity and physical characteristics. Factors such as growth, resilience to changes, disease resistance, and unique features contribute to the quality of Ziziphus species.
View Article and Find Full Text PDF