Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
We have engineered streptavidin labelled Europium doped fluorescent silica nanoparticles which significantly increased sensitivity without compromising the specificity of the immunoassay. As a proof of concept, a time resolved fluorescence based sandwich immunoassay was developed to detect HIV-1 p24 antigen in clinical specimens. The detection range of the silica nanoparticle based immunoassay (SNIA) was found to be between 0.02 to 500 pg/mL in a linear dose dependent manner. SNIA offers 1000 fold enhancement over conventional colorimetric ELISA. Testing of plasma samples that were HIV negative showed no false positive results in the detection of HIV-1 p24 antigen. This highly sensitive p24 assay can help improve blood safety by reducing the antibody negative window period in blood donors in resource limited settings where nucleic acid testing is not practical or feasible. This technology can also be easily transferred to a lab-on-a-chip platform for use in resource limited settings and can also be easily adopted for the detection of other antigens.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540927 | PMC |
| http://dx.doi.org/10.1038/s41598-017-07299-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Foliar Application of SiO Nanoparticles Altered Rhizosphere Metabolite and Microbe Profiles: Surface Chemistry-Driven Biological Effects.
J Agric Food Chem
September 2025
State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210023, China.
Silica nanoparticles (SiONPs), as emerging foliar nanofertilizers, demonstrate promising potential in agriculture. However, whether foliar application of SiONPs alters belowground soil metabolites and microbe composition and abundance remains largely unknown. In this study, 3-week-old cucumber plants were foliar-sprayed with fumed or Stöber SiO NPs dosing at -4 mg of NPs per plant for 5 days.
View Article and Find Full Text PDFA Mesoporous Silica-Based Naringenin Delivery System Promoting Macrophage M2 Polarization in Atherosclerosis.
Biomater Res
September 2025
Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Atherosclerosis is the leading cause of global cardiovascular morbidity and mortality associated with inflammatory and immunological mechanisms. Immunotherapy has demonstrated promising efficacy in the management of atherosclerosis. Nevertheless, certain immunotherapeutic approaches are associated with limitations, including suboptimal efficacy and non-negligible adverse effects.
View Article and Find Full Text PDFNanotechnology-driven strategies for tilapia vaccines: Comparative evaluation of nanoemulsions and silica nanoparticles against .
Vet World
July 2025
Research Center for Veterinary Science, National Research and Innovation Agency, Jl. Raya Bogor Km. 46 Cibinong, Bogor, 16911, West Java, Indonesia.
Streptococcosis, caused by , is a significant disease in tilapia farming that results in substantial economic losses. While vaccination is the most effective method for prevention, current vaccines face challenges when administered orally or through immersion, primarily due to poor absorption and degradation in the fish's digestive system. Nanotechnology offers new ways to improve vaccine delivery and effectiveness.
View Article and Find Full Text PDFFluorescent mesoporous silica nanoparticles for targeted tebuconazole delivery against brown root rot in rubber trees.
Plant Dis
September 2025
Hainan University, Haikou, Hainan, China;
Brown root rot, caused by Phellinus noxius, is a major threat to rubber tree cultivation, resulting in substantial economic losses. Traditional control methods, such as root irrigation with fungicides, are labor-intensive, water-consuming, and inefficient, particularly in regions with limited water resources. This study introduces fluorescent mesoporous silica nanoparticles (FL-MSNs) as a novel delivery platform for tebuconazole to target P.
View Article and Find Full Text PDFEnzyme-mediated nanoreactors with cascade metabolic modulation for enhanced chemo-chemodynamic combination therapy.
J Colloid Interface Sci
September 2025
School of Materials, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China. Electronic address:
Harnessing the significant buildup of lactic acid (LA) within the tumor microenvironment (TME) for metabolic manipulation presents a promising avenue for cancer treatment. Nevertheless, single-agent therapies often fail to address the complex and varying needs of TME heterogeneity, posing a substantial scientific hurdle in oncology. In this context, we employ asymmetric mesoporous silica nanoparticles (AMS NPs) as delivery vehicles, simultaneously loading them with zinc‑cobalt‑manganese ferrite nanoparticles (ZCMF NPs), lactate oxidase (LOX), and doxorubicin (DOX).
View Article and Find Full Text PDF