Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Pancreatic polypeptide (PP) is a specific biomarker of nonfunctional pancreatic neuroendocrine tumors (NF-pNETs). Clinical significance of PP inspires researchers to make great efforts in developing sensitive and specific sensors. However, there is no existing biosensor for detecting PP that combines facility and functionality. Addressing this challenge, a pair of aptamers which could be used to develop a sandwich assay for PP is reported. First, several high affinity aptamers are screened through graphene oxide-based SELEX, and appropriate dual-aptamers which could bind to different epitopes of PP are identified through fluorescence assays. Then the feasibility of the dual-aptamers for constructing the sandwich assay is validated via dynamic light scattering. This sandwich assay shows considerable sensitivity and specificity. The above results imply that the dual-aptamers have the potential toward developing novel sensors for PP in clinical samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acssensors.6b00836 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
"Sandwiched Individuals" Exploring the Push and Pull Factors Influencing Retention Among Mid-Career Nurses: An Integrative Review.
J Adv Nurs
September 2025
Nursing Department, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Aim: To systematically analyse international empirical literature and establish a comprehensive understanding of the push and pull factors influencing retention and turnover among mid-career nurses.
Design: An integrative review.
Data Sources: PubMed, Web of Science, Scopus, EMBASE (Ovid), and CINAHL (EBSCO) were searched for studies published between January 2001 and November 2024.
Simultaneous determination and degradation of aflatoxin B1 using a covalent-organic framework initiated hydrogel and aptamer-based colorimetric sensing platform.
Mikrochim Acta
September 2025
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, China.
As the most dangerous mycotoxin, aflatoxin B1 (AFB1) has caused some food safety issues to be concerned. In this study, a simultaneous detection and degradation method towards AFB1 was established. Covalent-organic frameworks (COFs) were firstly synthesized and directly in situ deposited on the stainless-steel mesh, which would trigger the free-radical polymerization of acrylamide to form a hydrogel coating.
View Article and Find Full Text PDFPrevalence, characterization, and transmissible factors of foodborne pathogens in the Al-Qassim Region, Saudi Arabia.
Cell Mol Biol (Noisy-le-grand)
September 2025
Department of Public Health, College of Applied Medical Sciences, Qassim University, Buraydah, 51452 P.O. Box 6666, Saudi Arabia.
Foodborne illnesses pose a significant public health threat globally, particularly in Saudi Arabia, where the rapid growth of the food service sector has increased the risk of exposure to multidrug-resistant (MDR) bacteria. Traditional microbiological methods are often time-consuming and may lack precision, highlighting the need for faster and more accurate diagnostic alternatives. In this study, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) was employed for the rapid and precise identification of bacterial contaminants in ready-to-eat (RTE) foods, alongside an assessment of their antibiotic resistance profiles.
View Article and Find Full Text PDFSERS technology in virus Detection: Advances, challenges, and future perspectives.
Biosens Bioelectron
August 2025
State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD), College of Pharmacy, Harbin Medical University, Heilongjiang, 150081, China; Research Unit of Health Sciences and Technology (HST), Faculty of Medicine University of Oulu, Finland; Heilongjiang Eye Hospital, Harbin, 150001, China
SERS has revolutionized viral detection with its high sensitivity and specificity. This review comprehensively explores the application progress, challenges, and future directions of SERS in viral detection. Firstly, the fundamental principles of SERS are introduced.
View Article and Find Full Text PDFComparative Analysis of Vaginal Inflammatory Cytokines Among HPV-Positive and HPV-Negative Women in Manila, Philippines.
Am J Reprod Immunol
September 2025
Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.
Problem: Human papillomavirus (HPV) is considered the necessary cause of cervical cancer. Studies showed that disruption of the inflammatory milieu in the cervicovaginal mucosa can promote the persistence of HPV, which can result in cervical carcinogenesis. This study determined sociodemographic factors and vaginal inflammatory cytokines associated with HPV infection in Manila, Philippines.
View Article and Find Full Text PDF