Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The yeast Saccharomyces cerevisiae is ideal for systematic studies relying on collections of modified strains (libraries). Despite the significance of yeast libraries and the immense variety of available tags and regulatory elements, only a few such libraries exist, as their construction is extremely expensive and laborious. To overcome these limitations, we developed a SWAp-Tag (SWAT) method that enables one parental library to be modified easily and efficiently to give rise to an endless variety of libraries of choice. To showcase the versatility of the SWAT approach, we constructed and investigated a library of ∼1,800 strains carrying SWAT-GFP modules at the amino termini of endomembrane proteins and then used it to create two new libraries (mCherry and seamless GFP). Our work demonstrates how the SWAT method allows fast and effortless creation of yeast libraries, opening the door to new ways of systematically studying cell biology.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4869835 | PMC |
| http://dx.doi.org/10.1038/nmeth.3795 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fluphenazine, an antipsychotic compound, ameliorates Alzheimer's disease by clearing amyloid beta accumulation in C. elegans.
Geroscience
September 2025
Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, USA.
The aging population worldwide faces an increasing burden of age-related conditions, with Alzheimer's disease being a prominent neurodegenerative concern. Drug repurposing, the practice of identifying new therapeutic applications for existing drugs, offers a promising avenue for accelerated intervention. In this study, we utilized the yeast Saccharomyces cerevisiae to screen a library of 1760 FDA-approved compounds, both with and without rapamycin, to assess potential synergistic effects on yeast growth.
View Article and Find Full Text PDFSlt2 positively regulates Myb-mediated cellulose utilization in .
mBio
September 2025
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Unlabelled: Fungal degradation of cellulose facilitates the sustainable harnessing of biosphere energy and carbon cycling. is one of the basidiomycetes with the largest number of hydrolytic enzymes in its genome. The mycelium of degrades cellulose through the production of substantial amounts of cellulase, enabling the absorption of carbon sources and nutrients essential for fruiting body development.
View Article and Find Full Text PDFT cell receptor (TCR) specificity is central to the efficacy of T cell therapies, yet scalable methods to map how TCR sequences shape antigen recognition remain limited. To address this, we introduce VelociRAPTR, a library-on-library approach that combines yeast-displayed TCR libraries with pMHC-displaying virus-like particles (pMHC-VLPs) to rapidly screen millions of TCR-antigen interactions. We show that pMHC-VLPs efficiently bind TCRs on yeast and generate equivalent data to recombinantly produced pMHC protein.
View Article and Find Full Text PDFLarge-Scale Expansion of the MALDI-TOF MS Library for Comprehensive Identification of Yeasts and Filamentous Fungi in Clinical and Sanitary Contexts.
Med Mycol J
August 2025
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center.
The rapid identification of microbes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is gaining attention, particularly in clinical laboratories and hygiene management in food manufacturing. However, unlike bacteria, technical issues related to preprocessing and a lack of comprehensive reference libraries pose challenges in fungi. In this study, we constructed a new MALDI-TOF MS database, named EMALiMB, that expands the existing reference library to accurately identify a wider range of microbial species.
View Article and Find Full Text PDFScreening for GmRCD1-Interacting Proteins in Glycine Max and Characterization of the GmRCD1-GmNAC058 Interaction.
Int J Mol Sci
August 2025
Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
In response to abiotic stress, plants utilize hub protein-mediated signaling networks, with members of the SIMILAR TO RCD ONE (SRO) protein family playing a pivotal role in regulating stress resistance pathways. This study investigates the functional role of the soybean GmRCD1 protein and its interaction mechanisms to elucidate its molecular regulatory network in stress resistance responses. By employing yeast two-hybrid technology to screen a soybean cDNA library under high-salt stress conditions, 17 potential interacting proteins were identified, which include NAC transcription factors (e.
View Article and Find Full Text PDF