Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Two-component signal transduction systems mediate adaptation to environmental changes in bacteria, plants, fungi, and protists. Each two-component system consists of a sensor histidine kinase and a response regulator. Chloroplast sensor kinase (CSK) is a modified sensor histidine kinase found in chloroplasts-photosynthetic organelles of plants and algae. CSK regulates the transcription of chloroplast genes in response to changes in photosynthetic electron transport. In this study, the full-length and truncated forms of Arabidopsis CSK proteins were overexpressed and purified in order to characterise their kinase and redox sensing activities. Our results show that CSK contains a modified kinase catalytic domain that binds ATP with high affinity and forms a quinone adduct that may confer redox sensing activity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054060 | PMC |
| http://dx.doi.org/10.1007/s11120-016-0229-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Liquid Crystal Sensor Harnessing a Biomimetic Acetylcholinesterase Nanozyme for Detection of Trypsin in Human Serum.
Anal Chem
September 2025
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan 250014, China.
Abnormal levels of trypsin in the human body can lead to various diseases, yet conventional detection methods often lack operational simplicity and real-time readout capabilities. This work presents a state-of-the-art metal organic framework (MOF) nanozyme-integrated liquid crystal (LC) sensor (MHN-LC sensor) and demonstrates the detection of trypsin as a proof of the concept. By rational engineering of the MOF-808 framework with Al and l-histidine coordination, a novel MOF nanozyme (MHis-NE) exhibiting exceptional acetylcholinesterase (AChE)-mimetic activity is successfully prepared.
View Article and Find Full Text PDFMycobacterial MutT1-mediated dephosphorylation of the sensor histidine kinases reveals a new link in the regulation of the two-component signaling.
Nucleic Acids Res
August 2025
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Bacterial pathogens such as Mycobacterium tuberculosis majorly rely on two-component signaling (TCS) systems to sense and generate adaptive responses to the dynamic and stressful host environment. TCS comprises a sensor histidine kinase (SHK) that perceives the environmental signal and a response regulator (RR) that modulates the target gene expression. TCS occurs via a phosphotransfer event from SHK to RR.
View Article and Find Full Text PDFUnlabelled: Signal transduction by histidine kinases (HKs) is nearly ubiquitous in bacterial species. HKs can either sense ligands directly or indirectly via a cognate solute binding protein (SBP). The molecular basis for SBP-dependent signal reception, however, remains poorly understood in most cases.
View Article and Find Full Text PDFDual-Probe Strategy-Enabled Multiplex Photoelectrochemical Sensor for Highly Sensitive Screening of Diabetes and Related Complications.
Anal Chem
September 2025
Hubei Key Laboratory of Plasma Chemistry and Advanced Materials, Hubei Engineering Technology Research Center of Optoelectronic and New Energy Materials, Wuhan Institute of Technology, Wuhan 430205, P. R. China.
Photoelectrochemical (PEC) biosensing has emerged as a vital tool in disease surveillance and therapeutic monitoring. However, most current PEC platforms are constrained to single biomarker detection, limiting their utility in comprehensive disease management. In this study, we report the development of a dual-target PEC biosensor by integrating silane molecules and β-cyclodextrin (β-CD) with carbon nitride materials, specifically designed for monitoring diabetes and its associated complication, uremia.
View Article and Find Full Text PDFEnhancement and mechanism of in-cluster two-component regulatory factors on granaticin production in Streptomyces vilmorinianum YP1.
J Biotechnol
August 2025
School of Food and Health, Beijing Technology and Business University, Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), Beijing 100048, China. Electronic address:
Granaticins, a class of bioactive benzoisochromanequinones (BIQs), are natural blue pigments with biological activities, including antibacterial properties and promising clinical anticancer applications. However, their clinical and industrial applications are limited by low production yields and unclear biosynthetic regulation. In this study, we identified a rare in-cluster two-component system (TCS; orf10/orf11) within the granaticin biosynthesis-related gene cluster (BGC) of Streptomyces vilmorinianum YP1, a novel high-yielding producer.
View Article and Find Full Text PDF