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Premise Of The Study: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners.
Methods And Results: Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis × A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy.
Conclusions: These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit.
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http://dx.doi.org/10.3732/apps.1500049 | DOI Listing |
BMC Plant Biol
September 2025
National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang, 311300, China.
Background: The bamboo species Dendrocalamus × mutatus T.P.Yi & B.
View Article and Find Full Text PDFInt J Mol Sci
July 2025
College of Life Sciences, Nanjing University, Nanjing 210023, China.
In the traditional classification system of the Lauraceae family based on morphology and anatomy, the phylogenetic position of the genus has long been controversial. Chloroplast (cp) evolution of has not yet been illuminated. In this study, we first sequenced and assembled the complete cp genomes of , and conducted the comparative cp genomics, phylogenomics, and divergence time estimation of this ecological and economic important genus.
View Article and Find Full Text PDFFront Plant Sci
July 2025
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
The disjunctive distribution of between Taiwan and mainland China provides a unique framework for understanding phylogeographic patterns and evolutionary processes in relict tree species. This study investigated the genetic diversity, population structure, and phylogeographic history of using microsatellite and chloroplast DNA markers. Analysis of 249 samples from nine populations revealed that Taiwanese populations possess higher genetic diversity with significant inbreeding, while mainland Chinese populations showed lower diversity and inbreeding levels.
View Article and Find Full Text PDFBMC Genomics
August 2025
Key Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, Tibet University, Ministry of Education, Lhasa, 850000, China.
Background: Phlomoides rotata, an endemic Tibetan medicinal plant adapted to extreme alpine environments, faces conservation challenges due to habitat degradation and overharvesting. Despite its ecological and medicinal importance, its mitochondrial genome remains uncharacterized, limiting insights into its evolutionary adaptations and genomic architecture.
Results: We present the initial de novo assembly and annotation of the P.
Front Genet
July 2025
Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou, Jiangsu, China.
The mitochondrial genome of Erodium stephanianum (Geraniaceae) exhibits remarkable complexity revealed through nanopore sequencing, which has unveiled both chloroplast-to-mitochondrion gene transfer and extensive DNA rearrangements. We collected leaf samples from E. stephanianum in June 2023, subsequently extracting total DNA and sequencing the mitogenome using both Oxford Nanopore and Illumina technologies.
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