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Article Abstract
Two major challenges in proteomics are the large number of proteins and their broad dynamic range in the cell. We exploited the abundance-dependent Michaelis-Menten kinetics of trypsin digestion to selectively digest and deplete abundant proteins with a method we call DigDeAPr. We validated the depletion mechanism with known yeast protein abundances, and we observed greater than threefold improvement in low-abundance human-protein identification and quantitation metrics. This methodology should be broadly applicable to many organisms, proteases and proteomic pipelines.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531578 | PMC |
| http://dx.doi.org/10.1038/nmeth.2250 | DOI Listing |
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