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Article Abstract
We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518588 | PMC |
| http://dx.doi.org/10.1038/nmeth.2249 | DOI Listing |
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