Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Enzymes in lipid metabolism acquire and deliver hydrophobic substrates and products from within lipid bilayers. The structure at 2.55 Å of one isozyme of a constitutive enzyme in lipid A biosynthesis, LpxI from Caulobacter crescentus, has a novel fold. Two domains close around a completely sequestered substrate, UDP-2,3-diacylglucosamine, and open to release products either to the neighboring enzyme in a putative multienzyme complex or to the bilayer. Mutation analysis identifies Asp225 as key to Mg(2+)-catalyzed diphosphate hydrolysis. These structures provide snapshots of the enzymatic synthesis of a critical lipid A precursor.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562136 | PMC |
| http://dx.doi.org/10.1038/nsmb.2393 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acute Pain After Total Knee Arthroplasty: 2-Arachidonoylglycerol Tone and Endocannabinoid/Eicosanoid Crosstalk.
Anesth Analg
September 2025
From the Department of Anesthesiology.
Background: Total knee arthroplasty (TKA) is a surgical procedure that induces intense acute postoperative pain, but the mechanisms that amplify post-TKA pain remain incompletely understood. Endocannabinoids, such as 2-arachidonoylglycerol (2-AG), are endogenous lipids that can produce antinociceptive effects. However, hydrolysis of 2-AG by monoacylglycerol lipase (MAGL) generates arachidonic acid, the precursor to a host of eicosanoids that enhance pain.
View Article and Find Full Text PDFAn alternative route for β-hydroxybutyrate metabolism supports cytosolic acetyl-CoA synthesis in cancer cells.
Nat Metab
September 2025
Department of Metabolism and Nutritional Programming, Van Andel Institute, Grand Rapids, MI, USA.
Cancer cells are exposed to diverse metabolites in the tumour microenvironment that are used to support the synthesis of nucleotides, amino acids and lipids needed for rapid cell proliferation. In some tumours, ketone bodies such as β-hydroxybutyrate (β-OHB), which are elevated in circulation under fasting conditions or low glycemic diets, can serve as an alternative fuel that is metabolized in the mitochondria to provide acetyl-CoA for the tricarboxylic acid (TCA) cycle. Here we identify a non-canonical route for β-OHB metabolism that bypasses the TCA cycle to generate cytosolic acetyl-CoA.
View Article and Find Full Text PDFLipid oxidation driven olefinic aldehyde biosynthesis shapes aged aroma in Qingzhuan tea.
Food Chem X
August 2025
Key Laboratory of Tea Resources Comprehensive Utilization, Ministry of Agriculture and Rural Affairs, Hubei Qingzhuan Tea Engineering Research Centre, Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064, China.
This study systematically investigates lipid dynamics and their role in aroma formation during Qingzhuan tea (QZT) processing. Using UHPLC-MRM-MS/MS and GC-MS, we analyzed fatty acids (FAs) and oxidized fatty acids (OFAs) across seven processing stages, identifying 31 FAs and 55 OFAs. Polyunsaturated fatty acids (PUFAs), particularly -linolenic acid (C18:3) and linoleic acid (C18:2), dominated the lipid profiles (43.
View Article and Find Full Text PDFEvaluation of aroma characteristics of meat as affected by environmental ammonia.
Food Chem X
August 2025
Institute of Plant Nutrition and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning 110161, China.
Ammonia accumulation in aquaculture systems affects the sensory quality of , and mechanisms linking ammonia to aroma changes remain unclear. This study investigated how ammonia exposure in aquatic environments (0, 10.47, and 41.
View Article and Find Full Text PDFIn-depth investigation of the spatial variations of DOM components in sediments under the influence of cascade dams and their interactions with microbial communities.
J Environ Manage
September 2025
Key Lab of Basin Water Resource and Eco-Environmental Science in Hubei Province, Basin Water Environmental Research Department, Changjiang River Scientific Research Institute, No.23 Huangpu Road, Wuhan, 430010, PR China; Innovation Team for Basin Water Environmental Protection and Governance of Chan
Small cascade dams drive spatial divergence in the composition of dissolved organic matter (DOM) in local sediments. Taking Xixi River in the southeast of China, a representative small cascade-dammed watershed, as an example, this study explored the spatial variations of DOM components and its interactions with microbial communities under the influence of cascade dams. Results revealed that DOM composition differed significantly, i.
View Article and Find Full Text PDF