Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c2lc21096g | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cross-reactivities in conjugation reactions involving iron oxide nanoparticles.
Beilstein J Nanotechnol
August 2025
Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC H3A 0B8, Canada.
The preparation of multimodal nanoparticles by capping magnetic iron oxide nanoparticles (IONPs) with functional organic molecules is a major area of research for biomedical applications. Conjugation reactions, such as carbodiimide coupling and the highly selective class of reactions known as "click chemistry", have been instrumental in tailoring the ligand layers of IONPs to produce functional biomedical nanomaterials. However, few studies report the controls performed to determine if the loading of molecules onto IONPs is due to the proposed coupling reaction(s) employed, or some other unknown interaction with the IONP surface.
View Article and Find Full Text PDFUser Engagement With a Mobile Health App for People Living With HIV: Observational Study Based on an Engagement Evaluation Framework.
J Med Internet Res
September 2025
College of Nursing, Yonsei University, Seoul, Republic of Korea.
Background: Mobile health (mHealth) interventions can be effective for people living with HIV, who are sensitive to privacy breach risks. Understanding the perceived experiences of intervention participants can provide comprehensive insights into potential users and predict intervention effectiveness. Thus, it is necessary to plan engagement measurement and consider ways to enhance engagement during the app development phase.
View Article and Find Full Text PDFConcentration-Dependent Nanosheet and Nanotube Networks: Ice-Water Interface Control for Antifreeze Application.
ACS Appl Mater Interfaces
September 2025
Department of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
Cyclic peptides (CPs) are versatile building blocks whose conformational constraints foster ordered supramolecular architectures with potential in biomedicine, nanoelectronics, and catalysis. Herein, we report the development of biomimetic antifreeze materials by conjugating CPs bearing ice-binding residues to 4-arm polyethylene glycol (PEG) via click chemistry. The concentration-dependent self-assembly of these CP-PEG conjugates induces programmable morphological transitions, forming nanotube networks above the critical aggregation concentration (CAC) and two-dimensional nanosheet networks near the CAC.
View Article and Find Full Text PDFDevelopment and Implementation of an Ambulatory Orders-Based Ophthalmology Imaging Workflow.
J Imaging Inform Med
September 2025
Department of Radiology, University of Cincinnati, Cincinnati, OH, USA.
Background: Ocular imaging is essential to the diagnosis and management of eye disease, yet standardized imaging workflows remain underdeveloped in the eye care setting. This manuscript describes the design and implementation of an orders-based imaging workflow for ambulatory ophthalmology integrated with the electronic health record and enterprise imaging systems.
Methods: We developed a DICOM-compliant workflow for pediatric ophthalmology imaging that supports HL7 integration, DICOM modality worklists, and enterprise archive storage.
Modification of Amino-Functionalized Organic-Silica Hybrid Monoliths via a Catalyst-Free Amino-Yne Click Reaction for Capillary Liquid Chromatography.
Electrophoresis
September 2025
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, P. R. China.
A novel post-modification strategy was developed for rapid functionalization of monoliths through amino-yne click chemistry. This approach enabled the conjugation of activated alkynes onto amino-functionalized organic-silica hybrid monolith surfaces under mild, catalyst-free conditions. Systematic investigation of critical reaction parameters was conducted to optimize the post-modification process.
View Article and Find Full Text PDF