Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
We report the development of a new real-time polymerase chain reaction (PCR) detection system that uses oligonucleotide "tagged" PCR primers, a fluorophore-labeled "universal" detection oligonucleotides, and a complementary quenching oligonucleotide. The fluorescence signal decreases as PCR product accumulates due to the increase in detection/quencher hybrid formation as the tagged primer is consumed. We use plasmids containing the influenza A matrix gene and the porA and ctrA genes of Neisseria meningitidis as targets for developing the system. Cycle threshold (Ct) values were generated, and the sensitivity of the new system (dubbed "PrimRglo") compared favorably with the commonly used SYBR green and Taqman detection systems and, unlike the latter system, does not require the design of a new dual-labeled detection oligonucleotide for each new target sequence.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ab.2011.12.038 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Peroxisome proliferator-activated receptor pathway and key genes in cerebellar dysfunction induced by chronic intermittent alcohol exposure: a transcriptomic study.
Neurol Res
September 2025
Department of Neurology, The First Affiliated Hospital of Jiamusi University, Jiamusi, People's Republic of China.
Background: We conducted a transcriptomic analysis to examine cerebellar transcriptional changes in a mouse model of chronic intermittent alcohol exposure.
Methods: We established a mouse model of chronic intermittent alcohol exposure and conducted a cerebellar transcriptomic analysis. After identifying differentially expressed genes, we analyzed pathway enrichment using the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology.
Plasma small extracellular vesicles: Markers and mediators of atrial scar, in patients with atrial fibrillation.
Heart Rhythm
September 2025
Translational Cardiology Group, Health Research Institute, Santiago de Compostela, Spain; CIBERCV, Madrid, España. Electronic address:
Background: High % of low-voltage area (LVA), a surrogate of scar, is associated with atrial fibrillation (AF) recurrence after pulmonary vein isolation (PVI). Noninvasive biomarkers of LVA are a medical need for PVI decision.
Objective: We aimed to identify the proteome profile of plasma extracellular vesicles (EVs) associated with high % LVA, their cellular origin, and their regulation by hyperglycemia.
Electroacupuncture promotes myelin repair in the corpus callosum region of cerebral ischemic rats by modulating microglia activation and polarization.
Neurol Res
September 2025
Department of Human Anatomy, Wannan Medical College, Wuhu, China.
Background: Ischemic stroke can damage the cerebral white matter, resulting in myelin loss and neurological deficits. Moreover, microglial activation plays an important role in ischemic stroke; therefore, inhibiting microglial activation has become an effective therapeutic target for ischemic stroke.
Objective: This study aimed to investigate the effects of electroacupuncture (EA) on microglial activation and polarization, and the role of oligodendrocyte genesis in myelin reformation after ischemic stroke.
Relationship between biotransformation enzyme genes in polycyclic aromatic hydrocarbon metabolism, telomere length and missed abortion.
Int J Environ Health Res
September 2025
Department of Epidemiology, School of Public Health, Shanxi Medical University, Jinzhong, China.
The mechanism underlying the effects of Polycyclic aromatic hydrocarbons (PAHs) on missed abortion (MA) remains unclear. This study explored the relationship between PAHs exposure, telomere length (TL), metabolizing enzyme gene polymorphism, and MA in a case-control study with 253 pregnant women. A competitive enzyme-linked immunosorbent assay (ELISA) was used to quantify PAH-DNA adducts.
View Article and Find Full Text PDFBackground: To improve the molecular diagnostic yield for Aspergillus spp. from respiratory samples, we developed and evaluated a new DNA extraction method directly from respiratory samples combined with in-house Aspergillus real-time PCR.
Methods: We developed a method using beads and resin, where a sample is centrifuged to separate the supernatant and pellet.