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Article Abstract
The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228994 | PMC |
| http://dx.doi.org/10.1038/nmeth.1775 | DOI Listing |
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