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Article Abstract
We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables large-scale, multiplexed quantitative proteomics using isobaric tagging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205195 | PMC |
| http://dx.doi.org/10.1038/nmeth.1716 | DOI Listing |
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